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LAT 抗体 (pTyr226)

Name: LAT 抗体 (pTyr226)
SKU: ABIN967594
Availability: OutOfStock

LAT 适用: 人 WB, IHC (f) 宿主: 小鼠 Monoclonal J96-1238-58-93 unconjugated

Western blot analysis of LAT (pY226) in human T lymphocytes. (LAT 抗体 (pTyr226))

Cells treated with a phosphatase to eliminate all phosphorylation (LAT 抗体 (pTyr226))

LAT (pY226) staining on tonsil. (LAT 抗体 (pTyr226))

Western Blotting (WB) image for anti-Linker For Activation of T Cells (LAT) (pTyr226) antibody (ABIN967594) (LAT 抗体 (pTyr226))

4 images

(5 references)

产品编号 ABIN967594

发货至: 中国

Datasheet as PDF

抗原 See all LAT 抗体

LAT (Linker For Activation of T Cells (LAT))
抗原表位
21

19

15

8

8

7

7

4

4

3

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
pTyr226
适用
134

60

43

1
人
宿主
126

9

2

1
小鼠
Compatible Secondaries
克隆类型
129

9
单克隆
标记
68

6

4

4

4

4

4

4

4

3

3

3

3

3

3

3

3

3

3

3

3
This LAT antibody is un-conjugated
应用范围
76

46

39

39

16

13

10

9

4

2

1

1

1
Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

品牌

BD Pharmingen™

产品特性

1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

纯化方法

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

免疫原

Phosphorylated Human LAT Peptide

克隆位点

J96-1238-58-93

亚型

IgG1 kappa

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LAT 适用: 人 ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

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LAT 适用: 人 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated

anti-Linker For Activation of T Cells (LAT) (AA 33-227) antibody
LAT 适用: 大鼠 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated

anti-Linker For Activation of T Cells (LAT) (Tyr255) antibody
LAT 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated

限制

仅限研究用
状态

Liquid

浓度

0.5 mg/mL

缓冲液

Aqueous buffered solution containing ≤0.09 % sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C

储存方法

Store undiluted at 4°C.
Janssen, Zhang: "Adaptor proteins in lymphocyte activation." in: Current opinion in immunology, Vol. 15, Issue 3, pp. 269-76, (2003) (PubMed).

Samelson: "Signal transduction mediated by the T cell antigen receptor: the role of adapter proteins." in: Annual review of immunology, Vol. 20, pp. 371-94, (2002) (PubMed).

Zhu, Janssen, Zhang: "Minimal requirement of tyrosine residues of linker for activation of T cells in TCR signaling and thymocyte development." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 170, Issue 1, pp. 325-33, (2002) (PubMed).

Lin, Weiss: "Identification of the minimal tyrosine residues required for linker for activation of T cell function." in: The Journal of biological chemistry, Vol. 276, Issue 31, pp. 29588-95, (2001) (PubMed).

Paz, Wang, Clarke, Lu, Stokoe, Abo: "Mapping the Zap-70 phosphorylation sites on LAT (linker for activation of T cells) required for recruitment and activation of signalling proteins in T cells." in: The Biochemical journal, Vol. 356, Issue Pt 2, pp. 461-71, (2001) (PubMed).
抗原

LAT (Linker For Activation of T Cells (LAT))

别名

LAT (LAT 产品)

别名

LAT antibody, lat antibody, LAT1 antibody, pp36 antibody, linker for activation of T-cells antibody, linker for activation of T cells antibody, LAT antibody, lat antibody, Lat antibody

背景

Engagement of the T cell receptor (TCR) induces signal transduction pathways that enhance gene transcription and cellular proliferation and differentiation. TCR ligation results in the recruitment and activation of multiple protein tyrosine kinases (PTKs), including lck, fyn, and ZAP70. Adaptor proteins, such as Grb2 and SLP-76, relay the signal to downstream effector molecules. LAT (linker for activation of T cells) is a substrate of the activated ZAP70 and functions to bridge the activated TCR and its associated PTKs with tyrosine kinase substrates. LAT is expressed as 36- and 38-kDa forms that result from post-translational modification, and as a 42-kDa form that results from alternative splicing. LAT is an integral membrane protein that is phosphorylated at five tyrosine sites upon TCR ligation. Following phosphorylation, LAT binds a number of important signaling molecules, including Grb2, Vav, PLCgamma1, and the p85 subunit of PI3K. Multiple studies have shown that functional LAT is required for T lymphocyte activation and thymocyte development.
The J96-1238.58.93 monoclonal antibody recognizes the phosphorylated tyrosine 226 (pY226) of LAT, which is one of the phosphotyrosine sites required for binding Vav, Grb2, and Gads.

分子量

38 kDa

途径

TCR Signaling, Fc-epsilon Receptor Signaling Pathway