(2 references)-
- 抗原 See all Topoisomerase I (TOP1) 抗体
- Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Topoisomerase I antibody is un-conjugated
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应用范围
- Western Blotting (WB)
- 品牌
- BD Pharmingen™
- 产品特性
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1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 克隆位点
- C-21
- 亚型
- IgM
- Top Product
- Discover our top product TOP1 Primary Antibody
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anti-Topoisomerase (DNA) I (TOP1) (AA 692-765) antibody
TOP1 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 1A1 unconjugated
anti-Topoisomerase (DNA) I (TOP1) antibodyTOP1 适用: 人 WB, IHC, IF, IP 宿主: 兔 Monoclonal unconjugated
anti-Topoisomerase (DNA) I (TOP1) antibodyTOP1 适用: 人 WB, ELISA, IHC, IP, FACS 宿主: 兔 Monoclonal 6D8 unconjugated Recombinant Antibody
anti-Topoisomerase (DNA) I (TOP1) antibodyTOP1 适用: 人, 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Topoisomerase (DNA) I (TOP1) (Internal Region) antibodyTOP1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Topoisomerase (DNA) I (TOP1) (AA 1-100) antibodyTOP1 适用: 人 WB, IHC, IP 宿主: 兔 Polyclonal unconjugated
anti-Topoisomerase (DNA) I (TOP1) (N-Term) antibodyTOP1 适用: 人 WB, FACS 宿主: 小鼠 Monoclonal 1291CT875-142-166 unconjugated
anti-Topoisomerase (DNA) I (TOP1) antibodyTOP1 适用: 人, 大鼠 WB, FACS 宿主: 小鼠 Monoclonal 1C6 unconjugated
anti-Topoisomerase (DNA) I (TOP1) (AA 358-501) antibodyTOP1 适用: 人 ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Topoisomerase (DNA) I (TOP1) (AA 692-765) antibodyTOP1 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
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- 应用备注
- The C-21 antibody is recommended for western blot analysis (1-2 µg/ml). A-431 human epidermal carcinoma cells (ATCC CRL-1555) or K562 human leukemia cells (ATCC CCL-243) may be used as a positive control for this application.
- 说明
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Related Products: ABIN967389, ABIN968533
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 4°C.
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: "DNA topoisomerase I: customs officer at the border between DNA and RNA worlds?" in: Journal of molecular medicine (Berlin, Germany), Vol. 75, Issue 11-12, pp. 786-800, (1998) (PubMed).
: "Decreased expression of DNA topoisomerase I in camptothecin-resistant tumor cell lines as determined by a monoclonal antibody." in: Cancer research, Vol. 50, Issue 21, pp. 6925-30, (1990) (PubMed).
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: "DNA topoisomerase I: customs officer at the border between DNA and RNA worlds?" in: Journal of molecular medicine (Berlin, Germany), Vol. 75, Issue 11-12, pp. 786-800, (1998) (PubMed).
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- 抗原
- Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))
- 别名
- DNA Topoisomerase I (TOP1 产品)
- 物质类
- Viral Protein
- 背景
- DNA damage may be caused by various environmental factors, including radiation, mutagenic chemicals and copy errors which occur during DNA replication. The correction of DNA damage, so called proofreading functions of the cell, is achieved by numerous excision-repair enzymes. Topoisomerases alter the helical structure of DNA by introducing the transient breaking and rejoining of DNA strands, allowing other excision-repair enzymes to correct DNA errors. Topoisomerase I (Topo I) is a ubiquitous, soluble enzyme whose expression is fairly constant throughout the cell cycle. The related enzyme, Topo II, is a primarily insoluble structural protein whose expression varies between cell types and during the cell cycle. In addition to its role in DNA mismatch repair, Topo I displays kinase activity, phosphorylating serine-arginine rich (SR) splicing factors, and perhaps regulating gene expression by changing the splicing pattern of structural genes. DNA Topo I migrates at a molecular weight of 100 kDa in SDS-PAGE. Clone C-21 recognizes human DNA Topoisomerase I. The antibody is routinely tested by western blot analysis of A-431 cell lysates.
- 分子量
- 100 kDa
- 途径
- Caspase Cascade in Apoptosis, Stem Cell Maintenance
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