PIK3R1 抗体
(2 references)-
- 抗原 See all PIK3R1 (PI3K p85a) 抗体
- PIK3R1 (PI3K p85a) (Phosphoinositide 3 Kinase, p85 alpha (PI3K p85a))
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适用
- 人, Cow, 猴
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This PIK3R1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunoprecipitation (IP)
- 品牌
- BD Pharmingen™
- 交叉反应
- 人, 猴
- 产品特性
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1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- Recombinant cow p85alpha
- 克隆位点
- U15
- 亚型
- IgG1
- Top Product
- Discover our top product PI3K p85a Primary Antibody
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anti-Phosphoinositide 3 Kinase, p85 alpha (PI3K p85a) (AA 1-454) antibody
PI3K p85a 适用: 人 WB, ELISA, IP, PLA 宿主: 小鼠 Monoclonal 3A10 unconjugated
anti-Phosphoinositide 3 Kinase, p85 alpha (PI3K p85a) (pTyr607) antibodyPI3K p85a 适用: 人, 小鼠, 大鼠, Pig WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Phosphoinositide 3 Kinase, p85 alpha (PI3K p85a) (AA 159-388) antibodyPI3K p85a 适用: 人, 小鼠 WB, ELISA, IHC, FACS, ICC 宿主: 小鼠 Monoclonal 6G10 unconjugated
anti-Phosphoinositide 3 Kinase, p85 alpha (PI3K p85a) (AA 501-600) antibodyPI3K p85a 适用: 人, 小鼠, 大鼠, 兔 WB, ELISA, IHC (p), IF (p), FACS, IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Phosphoinositide 3 Kinase, p85 alpha (PI3K p85a) (AA 1-30), (N-Term) antibodyPI3K p85a 适用: 人, 大鼠 WB, IHC (p), IF, FACS 宿主: 兔 Polyclonal RB11634 unconjugated
anti-Phosphoinositide 3 Kinase, p85 alpha (PI3K p85a) (AA 159-388) antibodyPI3K p85a 适用: 人, 小鼠 WB, ELISA, IHC, FACS, IF/ICC 宿主: 小鼠 Monoclonal unconjugated
anti-Phosphoinositide 3 Kinase, p85 alpha (PI3K p85a) (pTyr607) antibodyPI3K p85a 适用: 人, 小鼠, 大鼠, 小鸡 WB, ELISA, IHC (p), IF 宿主: 兔 Polyclonal unconjugated
anti-Phosphoinositide 3 Kinase, p85 alpha (PI3K p85a) (AA 15-180) antibodyPI3K p85a 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Phosphoinositide 3 Kinase, p85 alpha (PI3K p85a) (AA 1-424) antibodyPI3K p85a 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated
anti-Phosphoinositide 3 Kinase, p85 alpha (PI3K p85a) antibodyPI3K p85a 适用: 小鼠, 大鼠 WB, IHC (p) 宿主: 小鼠 Monoclonal unconjugated
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- 应用备注
- Clone U15 was originally characterized by ELISA, immunoprecipitation, and western blot analysis. Applications include immunoprecipitation (1-2 µg/1x10^6 cells) and western blot analysis (1-2 µg/ml). In immunoprecipitation, U15 can bring down an active kinase. Jurkat human T cells (ATCC TIB-152) are suggested as a positive control.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 4°C.
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: "Specific phosphopeptide binding regulates a conformational change in the PI 3-kinase SH2 domain associated with enzyme activation." in: The EMBO journal, Vol. 12, Issue 2, pp. 795-802, (1993) (PubMed).
: "Type I phosphatidylinositol kinase makes a novel inositol phospholipid, phosphatidylinositol-3-phosphate." in: Nature, Vol. 332, Issue 6165, pp. 644-6, (1988) (PubMed).
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: "Specific phosphopeptide binding regulates a conformational change in the PI 3-kinase SH2 domain associated with enzyme activation." in: The EMBO journal, Vol. 12, Issue 2, pp. 795-802, (1993) (PubMed).
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- 抗原
- PIK3R1 (PI3K p85a) (Phosphoinositide 3 Kinase, p85 alpha (PI3K p85a))
- 别名
- pi 3-Kinase p85 alpha (PI3K p85a 产品)
- 别名
- PI3CG antibody, PI3K antibody, PI3Kgamma antibody, PIK3 antibody, AGM7 antibody, GRB1 antibody, p85 antibody, p85-ALPHA antibody, PI3KA antibody, AA414921 antibody, C530050K14 antibody, p50alpha antibody, p55alpha antibody, p85alpha antibody, wu:fb65b05 antibody, phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit gamma antibody, phosphoinositide-3-kinase regulatory subunit 1 antibody, phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit beta antibody, phosphoinositide-3-kinase, regulatory subunit 1 (alpha) antibody, PIK3CG antibody, PIK3R1 antibody, Pik3r1 antibody, Pik3cb antibody, pik3r1 antibody
- 背景
- Phosphatidylinositol 3-kinase (PI3-kinase) is a universal enzyme associated with receptor signaling pathways. It generates second messenger phospholipids by phosphorylating the D-3 position of inositol phospholipids including phosphatidylinositol (PI), PI-4-phosphate, and PI-4, 5-biphosphate. The enzyme exists as a heterodimer composed of regulatory 85 kDa (p85) and catalytic 110 kDa (p110) subunits. The p85 subunit contains two SH2 (src-homology 2) domains and an SH3 domain. The catalytic activity of the p110 subunit is stimulated when the p85 regulatory subunit binds, through its SH2 domains, to activated receptor and non-receptor tyrosine kinases. Two p85 isoforms have been described, p85a and p85ß. Both isoforms bind to activated receptors and each may be responsible for mediating a subset of PI3-kinase interactions. Clone U15 recognizes the p85 regulatory subunit of PI3 kinase (p85alpha). It reacts with human, monkey, and cow PI3 kinase. It does not cross-react with mouse or rat PI3 kinase. Recombinant cow p85alpha was used as immunogen. The epitope has been mapped to the inter-SH2 spacer region of p85alpha. The antibody will block lipids binding to this region.
- 分子量
- 85 kDa
- 途径
- TCR Signaling, Response to Growth Hormone Stimulus, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Hepatitis C, Protein targeting to Nucleus, VEGF Signaling, BCR Signaling, Warburg Effect
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