CAN 抗体 (AA 349-505)
(3 references)-
- 抗原 See all CAN 抗体
- CAN (Calcineurin (CAN))
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抗原表位
- AA 349-505
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CAN antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunoprecipitation (IP)
- 品牌
- BD Pharmingen™
- 交叉反应
- 人
- 产品特性
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1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- Human beta1 isoform of calcineurin A Subunit aa. 349-505
- 克隆位点
- G182-1847
- 亚型
- IgG1
- Top Product
- Discover our top product CAN Primary Antibody
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anti-Calcineurin (CAN) (AA 7-301) antibody
CAN 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Calcineurin (CAN) (AA 21-51) antibodyCAN 适用: 人, 小鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Calcineurin (CAN) (AA 21-51), (N-Term) antibodyCAN 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Applications include immunoprecipitation (1-2 µg/ml) and western blot analysis (1-2 µg/ml). Jurkat human T cells (ATCC TIB-152)2, HeLa human carcinoma cells (ATCC CCL-2), and A-431 human epidermal carcinoma cells (ATCC CRL-1555) are suggested as positive controls. A431 cell lysate (ABIN968533) and HeLa cell lysate (ABIN968535) are also available as ready-to-use positive western blot control.
- 说明
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Related Products: ABIN967389, ABIN968533, ABIN968535
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 4°C.
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: "Cain, a novel physiologic protein inhibitor of calcineurin." in: The Journal of biological chemistry, Vol. 273, Issue 29, pp. 18325-31, (1998) (PubMed).
: "Mapping of the immunophilin-immunosuppressant site of interaction on calcineurin." in: The Journal of biological chemistry, Vol. 269, Issue 19, pp. 14199-204, (1994) (PubMed).
: "Calcineurin phosphatase activity in T lymphocytes is inhibited by FK 506 and cyclosporin A." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 9, pp. 3686-90, (1992) (PubMed).
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: "Cain, a novel physiologic protein inhibitor of calcineurin." in: The Journal of biological chemistry, Vol. 273, Issue 29, pp. 18325-31, (1998) (PubMed).
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- 抗原
- CAN (Calcineurin (CAN))
- 别名
- Calcineurin (CAN 产品)
- 背景
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Calcineurin is a Ca2+- and calmodulin-dependent serine/threonine phosphatase which consists of two subunits. The A subunit (~61 kDa) is catalytic and has calmodulinbinding activity, the B subunit (~19 kDa), which is myristylated at its N-terminus, has Ca[2+]-binding activity. Calcineurin is expressed ubiquitously in eukaryotic cells, including yeast. The protein is highly expressed in mammalian brain, and has been implicated in a number of calcium-dependent pathways in the nervous system, including regulation of neurotransmitter release. Calcineurin also plays a key role in T cell receptor-mediated signal transduction pathways. Calcineurin dephosphorylates components of NF-AT (Nuclear Factor of Activated T cells), allowing NF-AT to translocate to the nucleus where it participates with AP-1 in activation of multiple cytokine and surface receptor genes. Inhibition of calcineurin by immunosuppressive agents such as cyclosporin A and FK506 correlates with the suppression of early T cell activation events. These immunosuppressive agents bind to specific intracellular proteins, called immunophilins, to form complexes that inhibit calcineurin’s phosphatase activity. In the presence of these complexes, NF-AT remains phosphorylated and thus cannot translocate to the nucleus. A novel protein, Cain (calcineurin inhibitor), has been described which displays a similar pattern of neuronal and brain expression and has been shown to bind and inhibit calcineurin activity in vivo.
G182-1847 recognizes the alpha and beta isoforms of the A subunit of calcineurin (~61 kDa). It reacts with an epitope in the autoinhibitory domain. It does not recognize the B subunit of calcineurin. A recombinant tag-fusion protein containing amino acids 349-505 of the human beta1 isoform of calcineurin A subunit was used as immunogen. - 分子量
- 61 kDa
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