mGluR1 alpha 抗体
mGluR1a
适用: 大鼠
WB, IHC (fro)
宿主: 小鼠
Monoclonal
G209-488
unconjugated
1 image
(3 references)-
- 抗原 See all mGluR1 alpha (mGluR1a) products
- mGluR1 alpha (mGluR1a) (Glutamate Receptor, Metabotropic 1 alpha (mGluR1a))
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适用
- 大鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This mGluR1 alpha antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 品牌
- BD Pharmingen™
- 产品特性
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1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- Recombinant Rat mGluR1 Fusion Protein
- 克隆位点
- G209-488
- 亚型
- IgG1
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anti-Glutamate Receptor, Metabotropic 1 alpha (mGluR1a) (AA 1092-1199) antibody
mGluR1a 适用: 大鼠, 小鼠 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Applications include western blot analysis (1-2 µg/ml) and immunohistochemical staining of frozen tissue sections. Rat brain is suggested as a positive control for this application.
- 说明
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Related Products: ABIN967389
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 4°C.
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: "Antibodies inactivating mGluR1 metabotropic glutamate receptor block long-term depression in cultured Purkinje cells." in: Neuron, Vol. 12, Issue 6, pp. 1245-55, (1994) (PubMed).
: "Distribution of the mRNA for a metabotropic glutamate receptor (mGluR1) in the central nervous system: an in situ hybridization study in adult and developing rat." in: The Journal of comparative neurology, Vol. 322, Issue 1, pp. 121-35, (1992) (PubMed).
: "Sequence and expression of a metabotropic glutamate receptor." in: Nature, Vol. 349, Issue 6312, pp. 760-5, (1991) (PubMed).
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: "Antibodies inactivating mGluR1 metabotropic glutamate receptor block long-term depression in cultured Purkinje cells." in: Neuron, Vol. 12, Issue 6, pp. 1245-55, (1994) (PubMed).
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- 抗原
- mGluR1 alpha (mGluR1a) (Glutamate Receptor, Metabotropic 1 alpha (mGluR1a))
- 别名
- mGluR1 alpha (mGluR1a 产品)
- 背景
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Glutamate is a major excitatory neurotransmitter in mammalian brain. Glutamatergic neurotransmission is mediated by a family of glutamate receptors that can be grouped into two classes, ionotropic (GluR) and metabotropic (mGluR) receptors. The metabotropic glutamate receptors consist of at least seven subtypes that can be divided into three groups on the basis of their sequence similarities, intracellular second messengers, and agonist selectivity’s. These groups are 1) mGluR1 and mGluR5, 2) mGluR2 and mGluR3, and 3) mGluR4, mGluR6 and mGluR7. mGluR1 and mGluR5 are coupled to the inositol phosphate/Ca[2+] signal transduction pathway, whereas the other five receptors are linked to the inhibition of the cAMP cascade. mRNA analysis shows that the seven receptors have different expression patterns in the central nervous system. For example, the highest level of mGluR1 mRNA expression is found in the cerebellar Purkinje cells. mGluR7 mRNA is moderately expressed in these cells, whereas the mRNA of the other five mGluRs is barely detectable. Three splice variants have been described for mGluR1, mGluR1alpha (145 kDa), mGluR1beta (97 kDa) and mGluR1c (97 kDa).
G209-488 recognizes rat mGluR1alpha. It does not cross-react with the other splice variants of mGluR1, mGluR1beta and mGluR1c. Additionally G209-488 does not crossreact with mGluR5, the most closely related mGluR family member. The antibody was characterized by western blot analysis using rat brain membranes, and by immunohistochemical analysis using frozen rat brain tissue sections. A full length recombinant rat mGluR1 fusion protein was used as immunogen. - 分子量
- 133 kDa
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