Nestin 抗体
(16 references)-
- 抗原 See all Nestin (NES) 抗体
- Nestin (NES)
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适用
- 大鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Nestin antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Fluorescence Microscopy (FM)
- 品牌
- BD Pharmingen™
- 产品特性
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1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3. Please refer to us for technical protocols. - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- Rat (E15) spinal cord extracts
- 克隆位点
- Rat 401
- 亚型
- IgG1
- Top Product
- Discover our top product NES Primary Antibody
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anti-Nestin (NES) (AA 178-399) antibody
NES 适用: 人 WB, IHC, ICC, IP 宿主: 小鼠 Monoclonal D4 unconjugated
anti-Nestin (NES) antibodyNES 适用: 人 WB, IHC, ELISA, FACS, IF 宿主: 小鼠 Monoclonal 2C2F7 unconjugated
anti-Nestin (NES) (AA 1484-1500) antibodyNES 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Nestin (NES) (AA 252-290), (N-Term) antibodyNES 适用: 人, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Nestin (NES) (AA 1389-1416) antibodyNES 适用: 人 WB, FACS, IF, IHC (p) 宿主: 兔 Polyclonal RB16253 unconjugated
anti-Nestin (NES) antibodyNES 适用: 小鼠 IHC, ICC 宿主: 大鼠 Monoclonal 7A3 unconjugated
anti-Nestin (NES) (AA 698-844) antibodyNES 适用: 人 IHC (f) 宿主: 小鼠 Monoclonal NES-2911 unconjugated
anti-Nestin (NES) (AA 1389-1416) antibodyNES 适用: 人 WB, IHC, ELISA, FACS, IF 宿主: 兔 Polyclonal unconjugated
anti-Nestin (NES) (AA 681-750) antibodyNES 适用: 人, 小鼠, 大鼠 ELISA, FACS, IF (p), IHC (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
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- 说明
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Related Products: ABIN967389
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 4°C.
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: "Phox2b expression in the taste centers of fish." in: The Journal of comparative neurology, Vol. 520, Issue 16, pp. 3633-49, (2013) (PubMed).
: "Identification of the Tctex-1 regulatory element that directs expression to neural stem/progenitor cells in developing and adult brain." in: The Journal of comparative neurology, Vol. 518, Issue 16, pp. 3327-42, (2010) (PubMed).
: "Perisomatic-targeting granule cells in the mouse olfactory bulb." in: The Journal of comparative neurology, Vol. 515, Issue 4, pp. 409-26, (2009) (PubMed).
: "Chemokine receptor expression by neural progenitor cells in neurogenic regions of mouse brain." in: The Journal of comparative neurology, Vol. 500, Issue 6, pp. 1007-33, (2007) (PubMed).
: "Glial fibrillary acidic protein (GFAP)-positive radial-like cells are present in the vicinity of proliferative progenitors in the nucleus tractus solitarius of adult rat." in: The Journal of comparative neurology, Vol. 501, Issue 3, pp. 353-68, (2007) (PubMed).
: "Fate of endogenous stem/progenitor cells following spinal cord injury." in: The Journal of comparative neurology, Vol. 498, Issue 4, pp. 525-38, (2006) (PubMed).
: "Examination of granule layer cell count, cell density, and single-pulse BrdU incorporation in rat organotypic hippocampal slice cultures with respect to culture medium, septotemporal position, and ..." in: The Journal of comparative neurology, Vol. 497, Issue 3, pp. 397-415, (2006) (PubMed).
: "Characterization of the human nestin gene reveals a close evolutionary relationship to neurofilaments." in: Journal of cell science, Vol. 103 ( Pt 2), Issue 1, pp. 589-97, (1993) (PubMed).
: "Monoclonal antibodies to a rat nestin fusion protein recognize a 220-kDa polypeptide in subsets of fetal and adult human central nervous system neurons and in primitive neuroectodermal tumor cells." in: The American journal of pathology, Vol. 143, Issue 1, pp. 258-68, (1993) (PubMed).
: "Expression of the class VI intermediate filament nestin in human central nervous system tumors." in: Cancer research, Vol. 52, Issue 19, pp. 5334-41, (1992) (PubMed).
: "Nestin expression in embryonic human neuroepithelium and in human neuroepithelial tumor cells." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 66, Issue 3, pp. 303-13, (1992) (PubMed).
: "Region-specific differentiation of the hippocampal stem cell line HiB5 upon implantation into the developing mammalian brain." in: Cell, Vol. 66, Issue 4, pp. 713-29, (1991) (PubMed).
: "CNS stem cells express a new class of intermediate filament protein." in: Cell, Vol. 60, Issue 4, pp. 585-95, (1990) (PubMed).
: "Identification of proteins that are developmentally regulated during early cerebral corticogenesis in the rat." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 9, Issue 12, pp. 4303-17, (1990) (PubMed).
: "Proliferation and differentiation of rat neuroepithelial precursor cells in vivo." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 8, Issue 4, pp. 1144-51, (1988) (PubMed).
: "Identification of major cell classes in the developing mammalian nervous system." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 5, Issue 12, pp. 3310-28, (1986) (PubMed).
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: "Phox2b expression in the taste centers of fish." in: The Journal of comparative neurology, Vol. 520, Issue 16, pp. 3633-49, (2013) (PubMed).
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- 抗原
- Nestin (NES)
- 别名
- Nestin (NES 产品)
- 别名
- paranemin antibody, NES antibody, AA166324 antibody, C78523 antibody, ESTM46 antibody, nestin antibody, nestin S homeolog antibody, NES antibody, nes.S antibody, Nes antibody, nes antibody
- 背景
- Multipotential stem cells in the neural tube of the developing embryo give rise to the different neuronal cell types of the brain. Nestin is an intermediate filament protein which is abundantly expressed in neuroepithelial stem cells early in embryogenesis, but is absent from nearly all mature central nervous system (CNS) cells. After its down-regulation, GFAP and neurofilaments are expressed in differentiated astrocytes and neurons, respectively. Initial studies showed that the mouse anti-rat nestin antibody (clone Rat 401) recognized transient radial glial cells and dividing neuroepithelial stem cells in the embryonic rat CNS. Reportedly, Rat 401 has been used to analyze nestin expression in the developing rat nervous system and in immortalized CNS precursor cell lines (e.g such as in E11 rat CNS stem cells, but lost by postnatal day 6 (P6) in spinal cord and by P21 in the cerebellum). Although not expressed in normal adult CNS cells, nestin has been reportedly to be detectable in a variety of CNS tumors, suggesting that these tumors share gene expression patterns with primitive, undifferentiated CNS cells. Rat 401 positive cells have been reported to be found throughout the developing neural tube, but not in the adult CNS. More than 90% of dissociated E11 neural tube cells have been reported to be Rat 401 positive. Rat 401 recognizes rat nestin and has been reported not to cross-react with human nestin. Due to differential tissue expression, this antibody may recognize nestin as a doublet within a range of 198-260 kD.
- 分子量
- 198-260 kDa
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