1. Since applications vary, each investigator should titrate the reagent to obtain optimal results. 2. Please refer to us for technical protocols. 3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
纯化方法
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
The G143-850 antibody recognizes vascular endothelial growth factor (VEGF) by western blotting. VEGF is a homodimeric heavily glycosylated protein. Human VEGF occurs in several molecular variants which arise by alternative splicing of the mRNA. Splice forms of VEGF differ in biological properties such as the receptor types which they recognize. Various forms of VEGF can be detected at MW 18-25 kDa in SDS-PAGE.