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GM-CSF 抗体

CSF2 适用: 人 WB, IP, Inhibition 宿主: 大鼠 Monoclonal BVD2-21C11 unconjugated
产品编号 ABIN967465
发货至: 中国
  • 抗原 See all GM-CSF (CSF2) 抗体
    GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))
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    大鼠
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    单克隆
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    This GM-CSF antibody is un-conjugated
    应用范围
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    Western Blotting (WB), Immunoprecipitation (IP), Blocking Antibody (Inhibition)
    品牌
    BD Pharmingen™
    产品特性
    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to us for technical protocols.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    4. Ficoll-Paque is a trademark of Amersham Biosciences Limited.
    5. Cy is a trademark of Amersham Biosciences Limited.
    纯化方法
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    免疫原
    Recombinant human GM-CSF
    克隆位点
    BVD2-21C11
    亚型
    IgG2a
    Top Product
    Discover our top product CSF2 Primary Antibody
  • 应用备注
    Blocking Control for Intracellular Staining:
    The purified BVD2-21C11 antibody (ABIN967465) can be used as a blocking control to demonstrate specificity of human GM-CSF staining by the PE-BVD2-21C11. To perform this control, the fixed/permeabilized cells (~ 1 million) can be incubated with 1 - 10 µg of unlabeled BVD2-21C11 antibody (ABIN967465) for 20 minutes at 4°C, prior to staining with PE-BVD2-21C11 antibody (eg, 0.1 - 0.5 µg mAb/ 1 million cells). The intracellular cytokine staining technique and use of blocking controls are described in detail by C. Prussin and D. Metcalfe.

    ELISA Detection:
    The biotinylated BVD2-21C11 antibody, is useful as a detection antibody for a sandwich ELISA for measuring human GM-CSF protein levels. Biotinylated BVD2-21C11 antibody can be paired with the purified BVD2-23B6 antibody as the capture antibody, with recombinant human GM-CSF as the standard. This ELISA pair is recommended primarily for measuring cytokine from experimental cell culture systems.
    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    0.5 mg/mL
    缓冲液
    Aqueous buffered solution containing ≤0.09 % sodium azide.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C
    储存方法
    Store undiluted at 4°C.
  • Abrams: "Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies." in: Current protocols in immunology / edited by John E. Coligan ... [et al.], Vol. Chapter 6, pp. Unit 6.20, (2008) (PubMed).

    Prussin, Metcalfe: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." in: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, (1996) (PubMed).

    Abrams, Roncarolo, Yssel, Andersson, Gleich, Silver: "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." in: Immunological reviews, Vol. 127, Issue 9-10, pp. 5-24, (1992) (PubMed).

    Kita, Ohnishi, Okubo, Weiler, Abrams, Gleich: "Granulocyte/macrophage colony-stimulating factor and interleukin 3 release from human peripheral blood eosinophils and neutrophils." in: The Journal of experimental medicine, Vol. 174, Issue 3, pp. 745-8, (1991) (PubMed).

    Bacchetta, de Waal Malefijt, Yssel, Abrams, de Vries, Spits, Roncarolo: "Host-reactive CD4+ and CD8+ T cell clones isolated from a human chimera produce IL-5, IL-2, IFN-gamma and granulocyte/macrophage-colony-stimulating factor but not IL-4." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 144, Issue 3, pp. 902-8, (1990) (PubMed).

  • 抗原
    GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))
    别名
    GM-CSF (CSF2 产品)
    别名
    GMCSF antibody, GM-CSF antibody, CSF2 antibody, Csfgm antibody, Gm-CSf antibody, MGI-IGM antibody, Gm-csf antibody, Gmcsf antibody, CSF antibody, colony stimulating factor 2 antibody, colony stimulating factor 2 (granulocyte-macrophage) antibody, CSF2 antibody, Csf2 antibody
    背景
    The BVD2-21C11 monoclonal antibody specifically binds to human Granulocyte/Macrophage - Colony Stimulating Factor (GM-CSF). Human GM-CSF is encoded by the CSF2 gene and is also known as Colony Stimulating Factor 2. GM-CSF is produced by activated T lymphocytes, macrophages, endothelial cells, fibroblasts, stromal cells and other cell types including B lymphocytes, mast cells, eosinophils, and osteoblasts. GM-CSF stimulates the survival, proliferation and/or differentiation of various cell types including neutrophils, eosinophils, macrophages, dendritic cells, megakaryocytes, erythroid cells, endothelial cells and their precursors. The immunogen used to generate the BVD2-21C11 hybridoma was recombinant human GM-CSF. The BVD2-21C11 antibody has been reported to crossreact with GM-CSF from the rhesus monkey. BVD2-21C11 is a neutralizing antibody.
    Synonyms: CSF2, Colony stimulating factor 2 (granulocyte-macrophage), CSF, GMCSF
    途径
    JAK/STAT Signaling, Cellular Response to Molecule of Bacterial Origin
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