p53 抗体
(5 references)-
- 抗原 See all p53 (TP53) 抗体
- p53 (TP53) (Tumor Protein P53 (TP53))
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适用
- 人, Cow
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This p53 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 品牌
- BD Pharmingen™
- 交叉反应
- 牛
- 产品特性
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1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- Human p53
- 克隆位点
- DO
- 亚型
- IgG2a
- Top Product
- Discover our top product TP53 Primary Antibody
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anti-Tumor Protein P53 (TP53) (pSer392) antibody
TP53 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Tumor Protein P53 (TP53) (AA 1-100) antibodyTP53 适用: 人 WB, IHC, IP 宿主: 兔 Polyclonal unconjugated
anti-Tumor Protein P53 (TP53) antibodyKO Validated TP53 适用: 人 WB, IF, IHC (p), IP, ICC, ChIP 宿主: 兔 Polyclonal unconjugated
anti-Tumor Protein P53 (TP53) (N-Term) antibodyTP53 适用: 人 WB, IF, IHC (p), IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Tumor Protein P53 (TP53) (AA 301-393) antibodyTP53 适用: 人, 小鼠, 大鼠, 山羊 WB, ELISA, FACS, IHC (p), ICC, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Tumor Protein P53 (TP53) (pSer33) antibodyTP53 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Tumor Protein P53 (TP53) (AA 94-201) antibodyTP53 适用: 人 WB, ELISA, IF, IHC (p), IP 宿主: 小鼠 Monoclonal 2C3 unconjugated
anti-Tumor Protein P53 (TP53) (AA 94-201) antibodyTP53 适用: 人 WB, ELISA, IHC (p), PLA, RNAi 宿主: 小鼠 Monoclonal 2C11 unconjugated
anti-Tumor Protein P53 (TP53) (acLys319) antibodyTP53 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Tumor Protein P53 (TP53) (pSer20) antibodyTP53 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Applications include immunoprecipitation (1-2 µg/1x10^6 cells), western blot analysis (2 µg/ml), and immunohistochemistry of frozen and formalin-fixed paraffin-embedded tissue sections (5-20 µg/ml). Positive control cell lines include COS-7 SV40 transformed monkey kidney cells (ATCC CRL-1651), SK-BR-3 human breast carcinoma cells (ATCC HTB-30), and A431 human vulval carcinoma cells (ATCC CRL-1555). Positive immunostaining is seen in a high proportion of breast and colon carcinomas. p53 staining is not typically detected in normal skin, brain, kidney, lung, stomach or breast tissue.
- 说明
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Related Products: ABIN967389, ABIN968533
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 4°C.
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: "Overexpression of p53 and prognosis in breast cancer." in: Cancer, Vol. 72, Issue 12, pp. 3641-7, (1994) (PubMed).
: "Expression of the p53 gene in Hodgkin's disease: dissociation between immunohistochemistry and clinicopathological data." in: Human pathology, Vol. 25, Issue 5, pp. 449-54, (1994) (PubMed).
: "Immunochemical analysis of the p53 oncoprotein in matched primary and metastatic human tumours." in: European journal of cancer (Oxford, England : 1990), Vol. 29A, Issue 6, pp. 881-6, (1993) (PubMed).
: "An immunochemical analysis of the human nuclear phosphoprotein p53. New monoclonal antibodies and epitope mapping using recombinant p53." in: Journal of immunological methods, Vol. 151, Issue 1-2, pp. 237-44, (1992) (PubMed).
: "Cancer. A deadly inheritance." in: Nature, Vol. 348, Issue 6303, pp. 681-2, (1991) (PubMed).
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: "Overexpression of p53 and prognosis in breast cancer." in: Cancer, Vol. 72, Issue 12, pp. 3641-7, (1994) (PubMed).
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- 抗原
- p53 (TP53) (Tumor Protein P53 (TP53))
- 别名
- p53 (TP53 产品)
- 背景
- The gene for the nuclear phosphoprotein p53 is the most commonly mutated gene yet identified in human cancers. Missense mutations occur in tumors of the colon, lung, breast, ovary, bladder and several other organs. The mutant p53 is overexpressed in a variety of transformed cells and wild-type p53 forms specific complexes with several viral oncogenes including SV40 large T, E1B from adenovirus, and E6 from human papilloma virus. Wild type p53 plays a role as a checkpoint protein for DNA damage during the G1/S-phase of the cell cycle. However, it is still unclear, whether point mutated forms of p53 are simple null mutants and/or dominant negatively acting proteins. DO-1 reacts with human wild-type and mutant p53. It cross-reacts with bovine p53 but does not cross-react with mouse or rat p53. DO-1 recognizes an epitope between amino acids 1 and 45 of all known forms of human p53. Human recombinant p53 protein was used as immunogen.
- 分子量
- 53 kDa
- 途径
- p53 Pathway, MAPK Pathway, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, Warburg Effect
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