(6 references)-
- 抗原 See all JNK1/2 products
- JNK1/2
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This JNK1/2 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunoprecipitation (IP)
- 品牌
- BD Pharmingen™
- 产品特性
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1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- Human JNK1 Fusion Protein
- 克隆位点
- G151-666
- 亚型
- IgG2a
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anti-JNK1/2 (AA 311-424) antibody
适用: 人, 小鼠, 大鼠 WB, IF (cc), IF (p), ELISA, IHC (fro), IHC (p) 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- G151-666 immunoprecipitates inactive JNK1 and JNK2 kinases, therefore the antibody is not recommended for in vitro kinase assays. G151-666 is most useful for detection of JNK2 or for detection of both JNK1 and JNK2 in the same assay. Clone G151-333 appears to be stronger for detection of JNK1 and is generally suggested for most applications involving JNK1. Clone G151-333 can be used to immunoprecipitate an active JNK1 kinase. HeLa cells (ATCC CCL-1) or NIH-3T3 mouse embryonic fibroblasts (ATCC CRL-1658) are suggested as a positive control for western blot analysis.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 4°C.
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: "jun-NH2-terminal kinase activation mediated by UV-induced DNA lesions in melanoma and fibroblast cells." in: Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research, Vol. 6, Issue 11, pp. 1437-46, (1996) (PubMed).
: "Complexes of p21RAS with JUN N-terminal kinase and JUN proteins." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 23, pp. 10585-9, (1995) (PubMed).
: "UV irradiation and heat shock mediate JNK activation via alternate pathways." in: The Journal of biological chemistry, Vol. 270, Issue 44, pp. 26071-7, (1995) (PubMed).
: "JNK1: a protein kinase stimulated by UV light and Ha-Ras that binds and phosphorylates the c-Jun activation domain." in: Cell, Vol. 76, Issue 6, pp. 1025-37, (1994) (PubMed).
: "NF-kappa B activation by ultraviolet light not dependent on a nuclear signal." in: Science (New York, N.Y.), Vol. 261, Issue 5127, pp. 1442-5, (1993) (PubMed).
: "Identification of an oncoprotein- and UV-responsive protein kinase that binds and potentiates the c-Jun activation domain." in: Genes & development, Vol. 7, Issue 11, pp. 2135-48, (1993) (PubMed).
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: "jun-NH2-terminal kinase activation mediated by UV-induced DNA lesions in melanoma and fibroblast cells." in: Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research, Vol. 6, Issue 11, pp. 1437-46, (1996) (PubMed).
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- 抗原
- JNK1/2
- 别名
- JNK1/JNK2 (JNK1/2 产品)
- 背景
- C-Jun NH2-terminal kinase (JNK) binds to the c-Jun terminal transactivation domain and phosphorylates it on Ser-63 and Ser-73. Phosphorylation enhances the transcriptional activity of c-Jun. The Ser-Pro-acidic sequence targeted by JNK kinase activity establishes it as a prolinedirected kinase related to the MAP kinases and cyclin/dependent kinases. JNK may act as a tumor promoter in response to UV-irradiation since its activity is potently stimulated by radiation. This has relevance to observations that c-Jun transcriptional activity is upregulated by UV irradiation. In addition to UV irradiation, JNK is also activated by some other forms of cellular stress, including heatshock. Both the JNK1 (46 kDa) and JNK2 (54 kDa) isozymes appear equally capable of binding to the c-Jun terminal transactivation domain following induction by UV irradiation or heatshock. G151-666 recognizes both the JNK1 and JNK2 isozymes of JNK1. A bacterially expressed fusion protein of human JNK1 was used as immunogen.
- 分子量
- 46 kDa (JNK1), 54 kDa (JNK2)
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