(4 references)-
- 抗原 See all TFE3 抗体
- TFE3 (Transcription Factor E3 (TFE3))
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适用
- 人, 小鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This TFE3 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunoprecipitation (IP)
- 品牌
- BD Pharmingen™
- 产品特性
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1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- TFE3-L Protein
- 克隆位点
- G138-312
- 亚型
- IgG1
- Top Product
- Discover our top product TFE3 Primary Antibody
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anti-Transcription Factor E3 (TFE3) (Internal Region) antibody
TFE3 适用: 人, 小鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Transcription Factor E3 (TFE3) antibodyTFE3 适用: 人 WB, IHC 宿主: 兔 Monoclonal unconjugated
anti-Transcription Factor E3 (TFE3) (AA 1-30), (N-Term) antibodyTFE3 适用: 人, 小鼠 WB, IF 宿主: 兔 Polyclonal RB20263 unconjugated
anti-Transcription Factor E3 (TFE3) (AA 1-110) antibodyTFE3 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Transcription Factor E3 (TFE3) (AA 1-575) antibodyTFE3 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Transcription Factor E3 (TFE3) (AA 1-30) antibodyTFE3 适用: 人, 小鼠 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Transcription Factor E3 (TFE3) (AA 166-254) antibodyTFE3 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Transcription Factor E3 (TFE3) (AA 324-373) antibodyTFE3 适用: 人, 小鼠, 大鼠, 犬, Cow, 豚鼠, 兔, 马, Pig, Hamster, 斑马鱼, Bat, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Transcription Factor E3 (TFE3) (N-Term) antibodyVerified TFE3 适用: 人 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Transcription Factor E3 (TFE3) (AA 503-552) antibodyTFE3 适用: 人, 小鼠, 大鼠, 犬, Cow, 豚鼠, 兔, 马, Pig, Hamster, Bat, 猴 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Applications include western blot analysis (0.25-1.0 µg/ml). WI-38 cells are suggested as a positive control. Other applications include gel shift (supershifts) and immunoprecipitation, which are not routinely tested. The antibody has been used to detect in vitro translated TFE3-L and TFE3-L, expressed as a recombinant protein in bacteria. In gel shift assays, using µE3 as a probe, this antibody supershifts the complex.
- 说明
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Related Products: ABIN967389, ABIN968553
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 4°C.
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: "Myc/Max and other helix-loop-helix/leucine zipper proteins bend DNA toward the minor groove." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 24, pp. 11779-83, (1993) (PubMed).
: "TFEC, a basic helix-loop-helix protein, forms heterodimers with TFE3 and inhibits TFE3-dependent transcription activation." in: Molecular and cellular biology, Vol. 13, Issue 8, pp. 4505-12, (1993) (PubMed).
: "TFE3: a helix-loop-helix protein that activates transcription through the immunoglobulin enhancer muE3 motif." in: Genes & development, Vol. 4, Issue 2, pp. 167-79, (1990) (PubMed).
: "A new DNA binding and dimerization motif in immunoglobulin enhancer binding, daughterless, MyoD, and myc proteins." in: Cell, Vol. 56, Issue 5, pp. 777-83, (1989) (PubMed).
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: "Myc/Max and other helix-loop-helix/leucine zipper proteins bend DNA toward the minor groove." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 24, pp. 11779-83, (1993) (PubMed).
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- 抗原
- TFE3 (Transcription Factor E3 (TFE3))
- 别名
- TFE3 (TFE3 产品)
- 别名
- RCCP2 antibody, RCCX1 antibody, TFEA antibody, bHLHe33 antibody, F830016E06Rik antibody, Tcfe3 antibody, Tfe-3 antibody, RGD1559642 antibody, cb873 antibody, zgc:109815 antibody, transcription factor binding to IGHM enhancer 3 antibody, transcription factor E3 antibody, transcription factor binding to IGHM enhancer 3 S homeolog antibody, transcription factor binding to IGHM enhancer 3a antibody, TFE3 antibody, Tfe3 antibody, tfe3.S antibody, tfe3a antibody
- 背景
- TFE3 is a ubiquitously expressed 50 kDa transcription factor containing both basic helix-loop-helix (bHLH) and leucine zipper (ZIP) motifs. It was initially identified as a protein which bound to the µE3 site in the immunoglobulin heavy chain enhancer. In addition, it binds to the MLTF/USF site in the adenovirus major late promoter. Upon binding as a dimer, it induces a minor groove-oriented bend in the DNA, similar to other HLH proteins such as TFEB, USF, myc, and max. Binding results in transcriptional activation and is mediated through the basic region located just N-terminal to the HLH domain. Clone G138-312 recognizes TFE3. The antibody was raised against a bacterially expressed TFE3-L protein consisting of 327 amino acids encoded by nucleotides 361-2469 of the cloned cDNA.
- 分子量
- 50 kDa
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