1. Since applications vary, each investigator should titrate the reagent to obtain optimal results. 2. Please refer to us for technical protocols. 3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
纯化方法
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
免疫原
NAMRU mouse mammary gland epithelial cell line NMuMG
CD138 is expressed at low density on cells in the normal bone marrow, therefore, it may be desirable to amplify staining by using a biotinylated second-step antibody followed by a bright third-step reagent, such as Streptavidin-PE. Other reported applications include western blot analysis using a cationic blotting membrane and a specific method, ELISA, and immunohistochemical staining of acetone-fixed frozen and paraffin-embedded sections.
The 281-2 antibody reacts with the core protein of CD138 (Syndecan-1), a cell-surface, integral membrane heparan sulfate- and chondroitin sulfate-containing proteoglycan that binds to interstitial extracellular matrix molecules. Syndecan-1 is predominantly expressed on epithelial cells, where its expression correlates with normal epithelial organization. It is also expressed on B lymphocytes at specific stages during their differentiation: precursor B cells in the bone marrow and antibody-secreting cells, including plasma cells, but not mature peripheral B cells. It is thus implicated in mediating B cell-matrix interactions. CD138 expression is also regulated during embryonic development, and the molecule shows a tissue- specific structural polymorphism resulting from different post-translational modifications. The 281-2 antibody may be used to detect the differently glycosylated forms, because it reacts with the core protein. Furthermore, the mAb detects the Syndecan-1 ectodomain which is cleaved from cell surfaces by a metalloproteinase. This antibody is routinely tested by flow cytometric analysis. Synonyms: Syndecan-1