NUMA1 抗体 (Cytoplasmic, Nuclear)
-
- 抗原 See all NUMA1 抗体
- NUMA1 (Nuclear Mitotic Apparatus Protein 1 (NUMA1))
-
抗原表位
- Cytoplasmic, Nuclear
-
适用
- 人
-
宿主
- 小鼠
-
克隆类型
- 单克隆
-
标记
- This NUMA1 antibody is un-conjugated
-
应用范围
- ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 特异性
- Reacts with an intracellular 228 kDa protein found inthe nucleus during interphase.
- 纯化方法
- Concentrated.
- 免疫原
- Live Ls 174T cells (colon carcinoma).
- 克隆位点
- 3-3A
- 亚型
- IgM
- Top Product
- Discover our top product NUMA1 Primary Antibody
-
-
- 应用备注
-
Immunohistochemistry: 1:50-1:100 on frozen and formalin-fixed paraffin-embedded tissue sections. (For paraffin sections high temp. antigen demasker, 10mM citrate, pH 6.0, is required, (boiling tissue for 10 mins, followed by cooling for 10-20 mins).
Western Blot 1:100-1:300.
Staining Procedure: This antibody can be used on frozen and formalin tissue sections. Formalin-fixed paraffin-embdded tissue sections require, boilng the tisssue in 10 mM citrate buffer, pH 6.0 for 10-20 mins. followed by cooling to Rt for 10-20 mins. This step is done prior to antibody. The antibody may be used at a dilution of 1:50-1:100 in IHC. The optimal conditions should be determined by the individual laboratory. - 说明
-
Recommended Positive Control: Tonsil, Thymus, Spleen
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- 0.2 mg/ml.
- 缓冲液
- 20 mM tris-borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein, pH 7.5
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
-
-
Cleavage of the nuclear matrix protein NuMA during apoptosis." in: Experimental cell research, Vol. 233, Issue 1, pp. 21-4, (1997) (PubMed).
: "
-
Cleavage of the nuclear matrix protein NuMA during apoptosis." in: Experimental cell research, Vol. 233, Issue 1, pp. 21-4, (1997) (PubMed).
-
- 抗原
- NUMA1 (Nuclear Mitotic Apparatus Protein 1 (NUMA1))
- 别名
- NuMA (NUMA1 产品)
- 别名
- NUMA antibody, 6720401E04Rik antibody, AA764025 antibody, AL022610 antibody, AU014979 antibody, NUMA1 antibody, NuMA antibody, SP-H antibody, numa antibody, numa1 antibody, nuclear mitotic apparatus protein 1 antibody, nuclear mitotic apparatus protein 1 S homeolog antibody, NUMA1 antibody, Numa1 antibody, numa1 antibody, numa1.S antibody
- 分子量
- 228 kDa
- 基因ID
- 4926
- 途径
- Caspase Cascade in Apoptosis, Regulation of Actin Filament Polymerization, M Phase
-