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- 抗原 See all GST 抗体
- GST (Glutathione S Transferase (GST))
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适用
- Schistosoma japonicum
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克隆类型
- 单克隆
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标记
- This GST antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA
- 特异性
- Ni-NTA purified recombinant GST expressed in E. Coli strain M15.
- 纯化方法
- Antibodies are purified by protein A affinity chromatography
- 亚型
- IgG1
- Top Product
- Discover our top product GST Primary Antibody
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- 应用备注
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Western Blot: Dilution 1: 2000- 5,000
ELISA: Proposed dilution 1: 10,000.
Determining optimal working dilutions by titration test. - 限制
- 仅限研究用
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- 储存条件
- -20 °C
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Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase." in: Gene, Vol. 67, Issue 1, pp. 31-40, (1988) (PubMed).
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Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase." in: Gene, Vol. 67, Issue 1, pp. 31-40, (1988) (PubMed).
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- 抗原
- GST (Glutathione S Transferase (GST))
- 别名
- Glutathione-S-Transferase (GST) (GST 产品)
- 背景
- Plasmid vectors for the expression of coding regions of eukaryotic genes in E. coliare in common usage, such expression vectors often encode hybrid fusion proteins containing part prokaryotic and part eukaryotic specified proteins. For instance, the pGEX.3X expression vector developed by Smith and Johnson allows for synthesis of fusion proteins between glutathionine-S-transferase (GST) and proteins encoded by inserted cDNA sequences. Antibodies derived from these GST fusion proteins are useful for checking protein expression both in plaques and on Western blots as well as for immunoaffinity purification of proteins expressed in E. coli.
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