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GFP 抗体 (N-Term)

Name: GFP 抗体 (N-Term)
Brand: antibodies-online
SKU: ABIN966201
Availability: OutOfStock

GFP 适用: Aequorea victoria IHC 宿主: 兔 Polyclonal unconjugated

(1 reference)

产品编号 ABIN966201

发货至: 中国

Datasheet as PDF

抗原 See all GFP 抗体

GFP (Green Fluorescent Protein (GFP))
抗原表位
16

15

10

8

4

3

3

3

2

2

1

1

1

1

1

1

1

1
N-Term
适用
168

15

11

9
Aequorea victoria
宿主
61

59

34

13

9

5

2

1
兔
Compatible Secondaries
克隆类型
110

69
多克隆
标记
79

11

10

10

5

4

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This GFP antibody is un-conjugated
应用范围
142

76

47

29

27

23

21

21

14

13

13

12

12

4

4

4

4

2

1

1

1

1

1
Immunohistochemistry (IHC)

纯化方法

Purified by antigen-specific affinity chromatography.

免疫原

Polyclonal antibody produced in rabbits immunizing with a synthetic peptide corresponding to N-terminal residues of Aequorea victoria GFP (Green fluorescent protein)

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应用备注

ELISA, Western blotting: 1µg/ml for 2hrs.

限制

仅限研究用
状态

Liquid

缓冲液

This antibody is stored in PBS, 50% glycerol

储存液

Sodium azide

注意事项

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C
Inouye, Noguchi, Sakaki, Takagi, Miyata, Iwanaga, Miyata, Tsuji: "Cloning and sequence analysis of cDNA for the luminescent protein aequorin." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 82, Issue 10, pp. 3154-8, (1985) (PubMed).
抗原

GFP (Green Fluorescent Protein (GFP))

别名

GFP (GFP 产品)

背景

GFP(Green fluorescent protein) is an energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin. It contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Xaa-N and Gly-(N+2), and oxidation of Tyr-(N+1) to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen. Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.