This Estrogen Receptor alpha antibody is un-conjugated
应用范围
Immunohistochemistry (IHC)
特异性
Estrogen Receptor- (Ab-104) antibody detects endogenous levels of total Estrogen Receptor- protein.
纯化方法
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
免疫原
The antiserum was produced against synthesized non-phosphopeptide derived from human Estrogen Receptor- around the phosphorylation site of serine 104 (S-V-SP-P-S).
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C
Medunjanin, Hermani, De Servi, Grisouard, Rincke, Mayer: "Glycogen synthase kinase-3 interacts with and phosphorylates estrogen receptor alpha and is involved in the regulation of receptor activity." in: The Journal of biological chemistry, Vol. 280, Issue 38, pp. 33006-14, (2005) (PubMed).
Rogatsky, Trowbridge, Garabedian: "Potentiation of human estrogen receptor alpha transcriptional activation through phosphorylation of serines 104 and 106 by the cyclin A-CDK2 complex." in: The Journal of biological chemistry, Vol. 274, Issue 32, pp. 22296-302, (1999) (PubMed).