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ABO, Blood Group A Antigen 抗体

适用: 人 IHC (p), IHC (fro), IHC (fp) 宿主: 小鼠 Monoclonal B45-1 unconjugated
产品编号 ABIN965677
发货至: 中国
  • 抗原
    ABO, Blood Group A Antigen
    适用
    宿主
    • 12
    小鼠
    克隆类型
    • 12
    单克隆
    标记
    • 11
    • 1
    非结合性
    应用范围
    • 7
    • 5
    • 5
    • 4
    • 2
    • 2
    • 2
    • 1
    • 1
    Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))
    特异性
    This antibody reacts with blood group substance A, subtype A1 and A2, on the cell membranes of epithelium, endothelium and red blood cells.
    纯化方法
    Concentrated.
    免疫原
    Synthetic trisaccharide, alpha-D-N-Acetyl-galactosamine-(1-3)-(alpha-L-Fuc(1-2))-beta-D-Gal.
    克隆位点
    B45-1
    亚型
    IgM
  • 应用备注
    Immunohistochemistry: 1:50.
    Staining Procedure: This antibody can be used on formalin-fixed, paraffin-embedded tissue sections. Prolonged fixation in buffered formalin can destroy the epitope. The antibody may be used at a dilution of 1:50 in IHC. It is recommended that this product be used on frozen tissue sections or specimens. The optimal conditions should be determined by the individual laboratory.
    说明

    Recommended Positive Control: Human Tissues

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    0.2 mg/ml.
    缓冲液
    20 mM tris-borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein, pH 7.5
    储存液
    Sodium azide
    注意事项
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C
  • Manimala, Roach, Li, Gildersleeve: "High-throughput carbohydrate microarray profiling of 27 antibodies demonstrates widespread specificity problems." in: Glycobiology, Vol. 17, Issue 8, pp. 17C-23C, (2007) (PubMed).

    Chapman, Allhoff, Proppe, Prout: "Use of monoclonal antibodies for the localization of tissue isoantigens A and B in transitional cell carcinoma of the upper urinary tract." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 31, Issue 4, pp. 557-61, (1983) (PubMed).

  • 抗原
    ABO, Blood Group A Antigen
    别名
    Blood Group Antigen A
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