Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
交叉反应
小鼠, 大鼠
交叉反应 (详细)
No cross reactivity with other proteins.
产品特性
Rabbit IgG polyclonal antibody for Actin, cytoplasmic 2 (ACTG1) detection. Tested with WB, IHC-P in Human, Mouse, Rat.
免疫原
A synthetic peptide corresponding to a sequence at the C-terminal of human Actin , identical to the related mouse and rat sequences.< br/>Immunogen was affinity purified.
At -20 °C for one year. After reconstitution, at 4 °C for one month. It can also be aliquotted and stored frozen at -20 °C for a longer time.
Goebel, Brockmann, Bönnemann, Warlo, Hanefeld, Labeit, Durling, Laing: "Actin-related myopathy without any missense mutation in the ACTA1 gene." in: Journal of child neurology, Vol. 19, Issue 2, pp. 149-53, (2004) (PubMed).
Laing, Clarke, Dye, Liyanage, Walker, Kobayashi, Shimakawa, Hagiwara, Ouvrier, Sparrow, Nishino, North, Nonaka: "Actin mutations are one cause of congenital fibre type disproportion." in: Annals of neurology, Vol. 56, Issue 5, pp. 689-94, (2004) (PubMed).
Gunning, Ponte, Okayama, Engel, Blau, Kedes et al.: "Isolation and characterization of full-length cDNA clones for human alpha-, beta-, and gamma-actin mRNAs: skeletal but not cytoplasmic actins have an amino-terminal cysteine that is subsequently ..." in: Molecular and cellular biology, Vol. 3, Issue 5, pp. 787-95, (1983) (PubMed).
Actin, a highly conserved protein, is a major component of both the cytoskeletal and contractile structures in the cell types. It varies in amount, being related to the type of differentiation and to the functional state of cells and tissues. The actins exhibit over 90 % sequence homology, but each isoform has a unique NH2-terminal sequence. The isoforms are comprised of three alpha-actin, one beta-actin, two gamma-actin. Because the amino acid sequence of the C-terminal is the same for almost all actins, this antibody has been raised using a synthetic peptide corresponding to the C-terminal 11 residues.