POLB 抗体 (C-Term)
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- 抗原 See all POLB 抗体
- POLB (Polymerase (DNA Directed), beta (POLB))
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抗原表位
- C-Term
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This POLB antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA
- Supplier Product No.
- 600-401-c65
- Supplier
- Rockland
- 原理
- POLB Antibody
- 交叉反应 (详细)
- This antibody reacts with overexpressed and endogenous POLß protein.
- 产品特性
- Synonyms: rabbit anti-POLB antibody, rabbit anti-DNA polymerase beta antibody, DNA polymerase ß, POLß, POL-B, POL-ß, pol-beta, beta-pol
- 纯化方法
- This product was affinity purified from monospecific antiserum by immunoaffinity chromatography.
- 过滤
- Sterile filtered
- 免疫原
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Immunogen: This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminus of the POLß protein.
Immunogen Type: Conjugated Peptide
- 亚型
- IgG
- Top Product
- Discover our top product POLB Primary Antibody
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- 应用备注
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Application Note: This affinity purified POLß antibody has been tested by ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 38.1 kDa in size corresponding to POLß by western blotting in the appropriate cell lysate or extract.
Western Blot Dilution: 0.5 μg/mL
ELISA Dilution: 1:50,000
Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.4 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- POLB (Polymerase (DNA Directed), beta (POLB))
- 别名
- POLB (POLB 产品)
- 背景
- Background: The POLß protein belongs to the DNA polymerase type-X family. It is a DNA polymerase involved in gap-filling DNA synthesis or base excision and repair in a stepwise distributive fashion unlike other DNA polymerases. It is required for DNA maintenance, replication, recombination, and drug resistance. It is normally found as a monomer, in the cytoplasm and it translocates to the nucleus upon DNA damage.
- 基因ID
- 5423
- NCBI登录号
- NP_002681
- UniProt
- P06746
- 途径
- DNA Damage Repair
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