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RRM2B 抗体 (N-Term)

Rockland 600-401-b67 RRM2B 适用: 人 WB, ELISA, IHC, IP, FM 宿主: 兔 Polyclonal unconjugated
Rockland
产品编号 ABIN964915
Supplier Product No.: 600-401-b67
发货至: 中国
  • 抗原 See all RRM2B 抗体
    RRM2B (Ribonucleotide Reductase M2 B (TP53 Inducible) (RRM2B))
    抗原表位
    • 15
    • 7
    • 3
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    • 2
    • 2
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    N-Term
    适用
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    宿主
    • 43
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    克隆类型
    • 43
    • 3
    多克隆
    标记
    • 19
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    This RRM2B antibody is un-conjugated
    应用范围
    • 39
    • 16
    • 13
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    • 1
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    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Fluorescence Microscopy (FM)
    Supplier Product No.
    600-401-b67
    Supplier
    Rockland
    原理
    RRM2B p53R2 Antibody
    交叉反应 (详细)
    Anti-Human RRM2B/p53R2 is directed against RRM2B and reacts with the RRM2B from human tissues. Based on sequence we expect this antibody to react as well with RRM2B from other species.
    产品特性
    Synonyms: rabbit anti-RRM2B p53R2 antibody, rabbit anti-RRM2B antibody, rabbit anti-p53R2 antibody, Ribonucleoside-diphosphate reductase subunit M2 B, TP53-inducible ribonucleotide reductase M2 B, p53-inducible ribonucleotide reductase small subunit 2-like protein
    过滤
    Sterile filtered
    免疫原

    Immunogen: Anti-Human RRM2B/p53R2 antibody was prepared by repeated immunizations with a synthetic peptide corresponding to a region near the N-terminus of human RRM2B1 protein. A residue of cysteine was added to facilitate coupling.

    Immunogen Type: Conjugated Peptide

    亚型
    IgG
    Top Product
    Discover our top product RRM2B Primary Antibody
  • 应用备注

    Immunohistochemistry Dilution: User Optimized

    Application Note: RRM2B/p53R2 antibody is tested in Western Blot and ELISA and suitable for IP, IHC, and IF. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 41 kDa in size corresponding to RRM2B1 by western blotting in the appropriate cell lysate or extract.

    Western Blot Dilution: 1 μg/mL

    Immunoprecipitation Dilution: User Optimized

    ELISA Dilution: 1:3,000

    IF Microscopy Dilution: User Optimized

    Other: User Optimized

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    0.82 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    有效期
    12 months
  • Xue, Liu, Wang, Liu, Chen, Jia, Hsie, Chen, Luh, Zheng, Yen: "Ribonucleotide reductase subunit M2B deficiency leads to mitochondrial permeability transition pore opening and is associated with aggressive clinicopathologic manifestations of breast cancer." in: American journal of translational research, Vol. 10, Issue 11, pp. 3635-3649, (2018) (PubMed).

    Chen, Tsou, Hu, Ma, Liu, Yen, Ann: "Autophagy induction causes a synthetic lethal sensitization to ribonucleotide reductase inhibition in breast cancer cells." in: Oncotarget, Vol. 7, Issue 2, pp. 1984-99, (2016) (PubMed).

    Kuo, Sy, Xue, Chi, Lee, Yen, Chiang, Chang, Chu, Yen: "RRM2B suppresses activation of the oxidative stress pathway and is up-regulated by p53 during senescence." in: Scientific reports, Vol. 2, pp. 822, (2013) (PubMed).

    Liu, Lai, Wang, Xue, Leora, Wu, Hu, Zhang, Kuo, Zhou, Zhang, Wang, Wang, Zhou, Nelson, Zheng, Zhang, Chu, Yen: "Ribonucleotide reductase small subunit M2B prognoses better survival in colorectal cancer." in: Cancer research, Vol. 71, Issue 9, pp. 3202-13, (2011) (PubMed).

    Wang, Liu, Xue, Zhang, Kuo, Hu, Zhou, Ann, Zhang, Yen: "Ribonucleotide reductase subunit p53R2 regulates mitochondria homeostasis and function in KB and PC-3 cancer cells." in: Biochemical and biophysical research communications, Vol. 410, Issue 1, pp. 102-7, (2011) (PubMed).

  • 抗原
    RRM2B (Ribonucleotide Reductase M2 B (TP53 Inducible) (RRM2B))
    别名
    RRM2B (RRM2B 产品)
    背景
    Background: RRM2B/p53-R2, or p53-inducible ribonucleotide reductase small subunit 2-like protein, is a member of a broad superfamily of ferritin-like di-iron-carboxylate proteins. The RRM2B protein is an enzyme that catalyzes the conversion of ribonucleotides to deoxyribonucleotides that are essential for DNA synthesis, and is found in all eukaryotes. RRM2B plays a pivotal role in cell survival by repairing damaged DNA in a p53/TP53-dependent manner. It supplies deoxyribonucleotides for DNA repair in cells arrested at G1 or G2. It contains an iron-tyrosyl free radical center required for catalysis, and forms an active ribonucleotide reductase (RNR) complex with RRM1 which is expressed both in resting and proliferating cells in response to DNA damage. It is a heterotetramer with a large (RRM1) subunit, and interacts with p53/TP53. The interaction with RRM1 occurs in response to DNA damage and results in its translocation from cytoplasm to nucleus. It is widely expressed at a high level in skeletal muscle and at a weak level in thymus, and expressed in epithelial dysplasias and squamous cell carcinoma. Defects in RRM2B are the cause of encephalomyopathic mitochondrial depletion syndrome with renal tubulopathy (EMDSRT). Mitochondrial DNA depletion syndrome (MDS) is a clinically heterogeneous group of disorders characterized by a reduction in mitochondrial DNA (mtDNA) copy number. The encephalomyopathic form with renal tubulopathy is presented with various combinations of hypotonia, tubulopathy, seizures, respiratory distress, diarrhea, and lactic acidosis.
    基因ID
    50484, 42544136
    UniProt
    Q7LG56
    途径
    p53 Pathway, Negative Regulation of intrinsic apoptotic Signaling
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