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- 抗原 See all VEGF 抗体
- VEGF (Vascular Endothelial Growth Factor (VEGF))
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This VEGF antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA
- 特异性
- This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. This antibody is specific for human VEGFA-121 protein. A BLAST analysis was used to suggest cross-reactivity with VEGFA-121 from human sources based on 100% homology with the immunizing sequence. Based on high to 100% homology, there is a chance of cross-reactivity to VEGFA-121 from a wide variety of animals. Cross-reactivity with VEGFA-121 from other sources has not been determined.
- 产品特性
- Vascular endothelial growth factor-A was originally isolated from tumor cells and referred to as Tumor Angiogenesis Factor. Although expressed at high levels in certain tumor-derived cells it is produced by a wide variety of cell types. In addition to stimulating vascular growth, vascular permeability, cell migration, and endothelial cell proliferation and growth, it may play a role in stimulating vasolidation via nitric oxide-dependent pathways and in inhibition of apoptosis. VEGF binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Alternative splicing of the mRNA for VEGF-A results in several isoforms of the protein being produced. Rat and bovine VEGF are one amino acid shorter than the human factor, and the bovine and human sequences show a homology of 95 percent. In contrast to other factors mitogenic for endothelial cells such as FGF-1, FGF-2 and PDGF, VEGF is synthesized as a precursor containing a typical hydrophobic secretory signal sequence of 26 amino acids. Glycosylation is not required for efficient secretion of VEGF. VEGF121 is acidic, freely secreted, and widely expressed. This isoform is produced by alternative promoter usage and alternative initiation. It starts at an alternative upstream CUG codon and is post-translationally processed to produce the secreted VEGF peptide and a N-terminal peptide N-VEGF. The unprocessed protein and the N-VEGF peptide may localize to the nucleus, the endoplasmic reticulum and the Golgi or the extracellular matrix.
- 纯化方法
- purified
- 免疫原
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This IgG fraction antibody was prepared from rabbit antiserum after repeated immunizations with mature length recombinant human VEGFA-121 protein produced in E.coli.
Immunogen Type: RecombinantProtein - 亚型
- IgG
- Top Product
- Discover our top product VEGF Primary Antibody
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anti-Vascular Endothelial Growth Factor (VEGF) (AA 27-120) antibody
VEGF 适用: 人, 小鼠, 大鼠, Pig, 山羊 WB, ELISA, IHC (p), FACS, IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Vascular Endothelial Growth Factor (VEGF) antibodyVEGF 适用: 人, 小鼠, 大鼠, Pig, 犬, Cow, 小鸡 WB, IHC (p), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Vascular Endothelial Growth Factor (VEGF) antibodyVEGF 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 小鼠 Monoclonal unconjugated
anti-Vascular Endothelial Growth Factor (VEGF) (C-Term) antibodyVEGF 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB11705 unconjugated
anti-Vascular Endothelial Growth Factor (VEGF) antibodyVEGF 适用: 人, 大鼠 WB, ELISA, IHC, FACS, IP, BR 宿主: 小鼠 Monoclonal A-4-6-1 unconjugated Recombinant Antibody
anti-Vascular Endothelial Growth Factor (VEGF) (AA 27-233) antibodyVEGF 适用: 人 WB, IHC, IF 宿主: 小鼠 Monoclonal 4E3 unconjugated
anti-Vascular Endothelial Growth Factor (VEGF) antibodyVEGF 适用: 人, 小鼠 WB, IP 宿主: 小鼠 Monoclonal 6B7 unconjugated
anti-Vascular Endothelial Growth Factor (VEGF) (full length) antibodyVEGF 适用: 人, 小鼠, Pig WB, IF 宿主: 小鸡 Polyclonal unconjugated
anti-Vascular Endothelial Growth Factor (VEGF) antibody (Biotin)VEGF 适用: 人 WB, ELISA 宿主: 兔 Polyclonal Biotin
anti-Vascular Endothelial Growth Factor (VEGF) antibody (HRP)VEGF 适用: 人 WB, ELISA 宿主: 兔 Polyclonal HRP
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- 应用备注
- This purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 14.7 Da in size corresponding to the mature human VEGFA-121 protein by western blotting in appropriate cell lysate or extract.
- 说明
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Gene Name: VEGFA
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 100 µL
- 浓度
- 1.0 mg/mL
- 缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
- 储存液
- Without preservative
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is six (6) months from date of opening.
- 有效期
- 6 months
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- 抗原
- VEGF (Vascular Endothelial Growth Factor (VEGF))
- 别名
- VEGF (VEGF 产品)
- 别名
- MVCD1 antibody, VEGF antibody, VPF antibody, Vegf antibody, Vegf120 antibody, Vegf164 antibody, Vegf188 antibody, Vpf antibody, VEGF-A antibody, VEGF164 antibody, eVEGF120 antibody, eVEGF164 antibody, vegf-a antibody, vegf antibody, vascular endothelial growth factor A antibody, vascular endothelial growth factor A L homeolog antibody, vascular endothelial growth factor antibody, vascular endothelial growth factor precursor antibody, VEGFA antibody, Vegfa antibody, vegfa.L antibody, VEGF antibody, vegf antibody
- 背景
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Vascular endothelial growth factor-A was originally isolated from tumor cells and referred to as Tumor Angiogenesis Factor. Although expressed at high levels in certain tumor-derived cells it is produced by a wide variety of cell types. In addition to stimulating vascular growth, vascular permeability, cell migration, and endothelial cell proliferation and growth, it may play a role in stimulating vasolidation via nitric oxide-dependent pathways and in inhibition of apoptosis. VEGF binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Alternative splicing of the mRNA for VEGF-A results in several isoforms of the protein being produced. Rat and bovine VEGF are one amino acid shorter than the human factor, and the bovine and human sequences show a homology of 95 percent. In contrast to other factors mitogenic for endothelial cells such as FGF-1, FGF-2 and PDGF, VEGF is synthesized as a precursor containing a typical hydrophobic secretory signal sequence of 26 amino acids. Glycosylation is not required for efficient secretion of VEGF. VEGF121 is acidic, freely secreted, and widely expressed. This isoform is produced by alternative promoter usage and alternative initiation. It starts at an alternative upstream CUG codon and is post-translationally processed to produce the secreted VEGF peptide and a N-terminal peptide N-VEGF. The unprocessed protein and the N-VEGF peptide may localize to the nucleus, the endoplasmic reticulum and the Golgi or the extracellular matrix.
Synonyms: Vascular endothelial growth factor A-121, VEGF-A121 cytokine, Vascular permeability factor, VPF-121, VEGFA-121 - 基因ID
- 7422, 6631029
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