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IL-6 抗体

Rockland 209-301-310 IL6 适用: 人 WB, ELISA, FACS, MA 宿主: 小鼠 Monoclonal 33A12-G9 unconjugated
Rockland
产品编号 ABIN964743
Supplier Product No.: 209-301-310
发货至: 中国
  • 抗原 See all IL-6 (IL6) 抗体
    IL-6 (IL6) (Interleukin 6 (IL6))
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    小鼠
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    单克隆
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    This IL-6 antibody is un-conjugated
    应用范围
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    Western Blotting (WB), ELISA, Flow Cytometry (FACS), Multiplex Assay (MA)
    Supplier Product No.
    209-301-310
    Supplier
    Rockland
    原理
    IL-6 Antibody
    交叉反应 (详细)
    This purified antibody detects recombinant and native IL-6 present in body fluids and cell supernatants in various assays (ie.IL-1 stimulated IL-6 production from fibroblasts).
    产品特性
    Synonyms: mouse Anti-IL-6 antibody, mouse anti-interleukin-6 antibody, HSF, Hybridoma growth factor, Hybridoma plasmacytoma growth factor, IFNB2, IL 6, IL6 protein, Interferon beta 2, Interleukin 6, BSF2, CDF
    纯化方法
    Protein A purified
    过滤
    Sterile filtered
    内毒素水平
    Low Endotoxin
    免疫原

    Immunogen: This Protein A purified IL-6 monoclonal antibody was produced in mouse by repeated immunizations with mature full length recombinant human IL-6 produced in E.coli followed by hybridoma development.

    Immunogen Type: Recombinant Protein

    克隆位点
    33A12-G9
    亚型
    IgG1 kappa
    Top Product
    Discover our top product IL6 Primary Antibody
  • 应用备注

    Flow Cytometry Dilution: 0.5 mg/mL

    Application Note: Anti-IL-6 antibody has been tested for use in ELISA, Flow Cytometry, and western blotting. Reactivity is also expected in neutralizations, radioimmunoassay and immunohistochemistry. The endotoxin content is estimated to be <10 pg/μl by the LAL method. By western blot a band approximately 23.7 kDa in size corresponding to native human IL-6 protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.

    Western Blot Dilution: 1:1000

    ELISA Dilution: 1:10,000

    Other: User Optimized

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    2.19 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Store IL 6 antibody at -20° C . For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    有效期
    12 months
  • 抗原
    IL-6 (IL6) (Interleukin 6 (IL6))
    别名
    IL6 (IL6 产品)
    背景
    Background: IL-6 is a secreted cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response and plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. IL-6 induces myeloma and plasmacytoma growth and induces nerve cells differentiation and acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. IL-6 also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. Anti-IL-6 antibody is ideal for investigators involved in Cancer, Neuroscience and Immunology research.
    基因ID
    3569
    NCBI登录号
    NP_000591
    UniProt
    P05231
    途径
    TLR signaling, Hormone Transport, Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Autophagy, Cell RedoxHomeostasis, Cancer Immune Checkpoints, Inflammasome
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