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OspB 抗体

Rockland 200-401-c15s 适用: Borrelia burgdorferi ELISA, WB 宿主: 兔 Polyclonal unconjugated
Rockland
产品编号 ABIN1043770
Supplier Product No.: 200-401-c15s
发货至: 中国
  • 抗原
    OspB
    适用
    Borrelia burgdorferi
    宿主
    • 1
    克隆类型
    • 1
    多克隆
    标记
    • 1
    非结合性
    应用范围
    ELISA, Western Blotting (WB)
    Supplier Product No.
    200-401-c15s
    Supplier
    Rockland
    原理
    OspB Antibody
    交叉反应 (详细)
    This antibody is specific for Borrelia burgdorferi OspB protein.
    产品特性
    Synonyms: rabbit anti-OspB Antibody, Outer surface protein B, Borrelia burgdorferi OspB, locus BB_A16
    纯化方法
    This product was Protein-A purified and cross-adsorbed against MBP from monospecific antiserum by chromatography.
    免疫原

    Immunogen: MBP-fusion protein corresponding to Borrelia burgdorferi OspB protein.

    Immunogen Type: Recombinant Protein

    亚型
    IgG
  • 应用备注

    Application Note: This protein-A purified antibody has been tested for use in ELISA and Western blotting. Specific conditions for reactivity should be optimized by the user. Expect a band approximately 30.3 kDa in size corresponding to Borrelia burgdorferi OspB protein by Western blotting in the appropriate cell lysate or extract.

    Western Blot Dilution: 1:1,000

    ELISA Dilution: 1:13,000

    Other: User Optimized

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    浓度
    1.1 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    有效期
    12 months
  • Neelakanta, Li, Pal, Liu, Beck, DePonte, Fish, Kantor, Fikrig: "Outer surface protein B is critical for Borrelia burgdorferi adherence and survival within Ixodes ticks." in: PLoS pathogens, Vol. 3, Issue 3, pp. e33, (2007) (PubMed).

    Liang, Caimano, Radolf, Fikrig: "Borrelia burgdorferi outer surface protein (osp) B expression independent of ospA." in: Microbial pathogenesis, Vol. 37, Issue 1, pp. 35-40, (2004) (PubMed).

    Haake: "Spirochaetal lipoproteins and pathogenesis." in: Microbiology (Reading, England), Vol. 146 ( Pt 7), pp. 1491-504, (2000) (PubMed).

  • 抗原
    OspB
    背景
    Background: OspB, is one of the major Outer Surface Proteins of the outer membrane of Borrelia burgdorferi, which is composed of various unique outer surface proteins (Osp) that have been characterized (OspA through OspF). The Osp proteins are lipoproteins anchored by N-terminally attached fatty acid molecules to the membrane. They are presumed to play a role in virulence, transmission, or survival in the tick. Two of the major surface Ag of Borrelia burgdorferi, the 31- kDa OspA and 34- kDa OspB proteins, show a high degree of sequence similarity, are encoded by a 49-kb plasmid and share a common promoter, and are coordinately transcribed. OspA, OspB, and OspD are expressed by B. burgdorferi residing in the gut of unfed ticks, suggesting that they promote the persistence of the spirochete in ticks between blood meals. OspB has a contributing role in the adherence of B. burgdorferi to the tick gut. The C terminus of OspB is important for eliciting a protective immune response to OspB. B. burgdorferi has the ability to vary its surface proteins in response to immune attack.
    基因ID
    1194340
    NCBI登录号
    WP_010890379
    UniProt
    P17739
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