OspA 抗体
This anti-OspA antibody is a 兔 多克隆 antibody detecting OspA in ELISA 和 WB. Suitable for Borrelia burgdorferi.
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Quick Overview for OspA 抗体 (ABIN964719)
抗原
OspA
(Borrelia Burgdorferi Outer Surface Protein A (OspA))
适用
Borrelia burgdorferi
宿主
兔
克隆类型
多克隆
标记
This OspA antibody is un-conjugated
应用范围
ELISA, Western Blotting (WB)
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Supplier Product No.
- 200-401-c13
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Supplier
- Rockland
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原理
- OspA Antibody
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交叉反应 （详细）
- This antibody is specific for Borrelia burgdorferi OspA protein.
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产品特性
- Synonyms: rabbit anti-OspA Antibody, Outer surface protein A, Borrelia burgdorferi OspA, ospA, BB_A15, Plasmid lp54
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纯化方法
- This product was Protein-A purified and cross-adsorbed against MBP from monospecific antiserum by chromatography.
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免疫原
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Immunogen: MBP-fusion protein corresponding to Borrelia burgdorferi OspA protein.
Immunogen Type: Recombinant Protein
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亚型
- IgG
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应用备注
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Application Note: This protein-A purified antibody has been tested for use in ELISA and Western blotting. Specific conditions for reactivity should be optimized by the user. Expect a band approximately 28.1 kDa in size corresponding to Borrelia burgdorferi OspA protein by Western blotting in the appropriate cell lysate or extract.
Western Blot Dilution: 1:1,000
ELISA Dilution: >1:5,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- OspA (Borrelia Burgdorferi Outer Surface Protein A (OspA))
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别名
- ospA
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物质类
- Bacteria Protein
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背景
- Background: Outer-Surface Protein A (OspA), a lipoprotein from Borrelia burgdorferi encoded on its Plasmid lp54, is a major component of the spirochete's extracellular matrix. OspA probably serves as a lipid-anchor. The spirochetes migrate from the tick midgut during feeding to its salivary glands and are thus transmitted to the mammal host. This transition may be facilitated by changes in expression of some B. burgdorferi genes. Upon transmission of the spirochete from the Ixodes tick to mammalian host, the transcript level of OspA can change. It is believed that expression of the various proteins associated with the spirochete may be regulated by the changes in tick life cycle, changes in conditions during tick feeding (such as temperature, pH , and nutrients) and/or in coordination with the course of infection of the mammal host. B. burgdorferi can attach to (and also differentially express antigens in) diverse tissues within the vertebrate host and the tick vector, suggesting that physiological factors other than pH and temperature may play roles in modulating B. burgdorferi gene expression.
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基因ID
- 1194357
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NCBI登录号
- NP_045688
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UniProt
- P0CL66
抗原
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