OspC 抗体
Rockland 200-401-c11
适用: Borrelia burgdorferi
ELISA, WB
宿主: 兔
Polyclonal
unconjugated
1 image
(4 references)
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- 抗原
- OspC
- 适用
- Borrelia burgdorferi
- 宿主
- 兔
- 克隆类型
- 多克隆
- 应用范围
- ELISA, Western Blotting (WB)
- Supplier Product No.
- 200-401-c11
- Supplier
- Rockland
- 原理
- OspC Antibody
- 交叉反应 （详细）
- This antibody is specific for Borrelia burgdorferi OspC protein.
- 产品特性
- Synonyms: rabbit anti-OspC Antibody, Borrelia burgdorferi OspC, PC, Outer Surface Protein C, BB_B19
- 纯化方法
- This product was Protein-A purified and cross-adsorbed against MBP from monospecific antiserum by chromatography.
- 免疫原
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Immunogen: MBP-fusion protein corresponding to Borrelia burgdorferi OspC protein.
Immunogen Type: Recombinant Protein
- 亚型
- IgG
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- 应用备注
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Application Note: This protein-A purified antibody has been tested for use in Western blotting and in ELISA. Specific conditions for reactivity should be optimized by the user. Expect a band approximately 20.7 kDa in size corresponding to Borrelia burgdorferi OspC protein by Western blotting in the appropriate cell lysate or extract.
Western Blot Dilution: 1:1,000
Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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: "Borrelia burgdorferi Outer Membrane Vesicles Contain Antigenic Proteins, but Do Not Induce Cell Death in Human Cells." in: Microorganisms, Vol. 10, Issue 2, (2022) (PubMed).
: "Paper-based multiplexed vertical flow assay for point-of-care testing." in: Lab on a chip, Vol. 19, Issue 6, pp. 1027-1034, (2019) (PubMed).
: "Borrelia burgdorferi outer surface protein C (OspC) binds complement component C4b and confers bloodstream survival." in: Cellular microbiology, Vol. 19, Issue 12, (2018) (PubMed).
: "A short-term Borrelia burgdorferi infection model identifies tissue tropisms and bloodstream survival conferred by adhesion proteins." in: Infection and immunity, Vol. 83, Issue 8, pp. 3184-94, (2015) (PubMed).
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: "Borrelia burgdorferi Outer Membrane Vesicles Contain Antigenic Proteins, but Do Not Induce Cell Death in Human Cells." in: Microorganisms, Vol. 10, Issue 2, (2022) (PubMed).
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- 抗原
- OspC
- 背景
- Background: Outer Surface Protein C, or OspC, is a 20.7 kDa immunogenic protein on the outer surface of the spirochete Borrelia burgdorferi. Its function is not known, but it is located with lipid-anchoring sites on the outer cell membrane.
- 基因ID
- 1194415
- NCBI登录号
- WP_010890595
- UniProt
- Q07337
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