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VEGFA 抗体

Rockland 200-401-b74 VEGFA 适用: Cow WB, ELISA 宿主: 兔 Polyclonal unconjugated
Rockland
产品编号 ABIN964713
Supplier Product No.: 200-401-b74
发货至: 中国
  • 抗原 See all VEGFA 抗体
    VEGFA (Vascular Endothelial Growth Factor A (VEGFA))
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    宿主
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    This VEGFA antibody is un-conjugated
    应用范围
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    Western Blotting (WB), ELISA
    Supplier Product No.
    200-401-b74
    Supplier
    Rockland
    原理
    VEGF-A Antibody
    交叉反应 (详细)
    This antibody is specific for bovine VEGF-A protein.
    产品特性
    Synonyms: rabbit anti-VEGF-A Antibody, Vascular endothelial growth factor A, VEGF A, VEGFA, Vascular permeability factor, VPF
    纯化方法
    This product was Protein-A purified from monospecific antiserum by chromatography.
    免疫原

    Immunogen: This protein-A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein raised in yeast, corresponding to the 164 amino acids of the mature bovine VEGF-A protein.

    Immunogen Type: Recombinant Protein

    亚型
    IgG
    Top Product
    Discover our top product VEGFA Primary Antibody
  • 应用备注

    Application Note: This protein-A purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 19.2 kDa in size corresponding to bovine VEGF-A protein by western blotting in the appropriate cell lysate or extract.

    Western Blot Dilution: 1:333

    ELISA Dilution: User Optimized

    Other: User Optimized

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    浓度
    1.0 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    有效期
    12 months
  • 抗原
    VEGFA (Vascular Endothelial Growth Factor A (VEGFA))
    别名
    VEGFA (VEGFA 产品)
    背景
    Background: VEGF is a potent mitogen in embryonic and somatic angiogenesis with specificity for vascular endothelial cells. VEGF forms homodimers and exists in four different isoforms. Overall, the VEGF monomer resembles that of PDGF, but its N-terminal segment is helical rather than extended. VEGF shares homologies of about 21 % and 24 % respectively with the A and B chains of human platelet-derived growth factor (PDGF), and has complete conservation of the eight cysteine residues found in both mature PDGF chains. The cysteine knot motif is a common feature of this domain. The homology is not reflected in function, however, since the cell types responsive to VEGF are distinct from those responsive to homo- and heterodimers of the PDGF chains. This protein is a glycosylated mitogen that acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. VEGF-A also has been shown to have effects on a number of other cell types (e.g. stimulation of monocyte/macrophage migration, neurons, cancer cells, kidney epithelial cells ). VEGF-A is also a vasodilator, it increases microvascular permeability, and was originally referred to as vascular permeability factor. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized.
    基因ID
    281572, 27806357
    UniProt
    P15691
    途径
    RTK signaling, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling
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