Streptavidin 抗体
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- 抗原 See all Streptavidin 抗体
- Streptavidin
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适用
- Streptomyces avidinii
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Streptavidin antibody is un-conjugated
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应用范围
- ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Dot Blot (DB)
- Supplier Product No.
- 200-401-095
- Supplier
- Rockland
- 原理
- Streptavidin Antibody
- 交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Streptavidin [Streptomyces avidinii].
- 产品特性
- Synonyms: rabbit anti-Streptavidin Antibody, rabbit anti Streptavidin
- 纯化方法
- Anti-Streptavidin antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
- 过滤
- Sterile filtered
- 免疫原
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Immunogen: Streptavidin [Streptomyces avidinii]
Immunogen Type: Native Protein
- 亚型
- IgG
- Top Product
- Discover our top product Streptavidin Primary Antibody
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anti-Streptavidin antibody
适用: 人 ELISA, WB 宿主: 小鼠 Monoclonal 1C2 unconjugated
anti-Streptavidin (C-Term) antibody适用: Streptomyces avidinii WB 宿主: 山羊 Polyclonal unconjugated
anti-Streptavidin (AA 25-183) antibody适用: Streptomyces avidinii ELISA, WB 宿主: 小鼠 Monoclonal 1C2 unconjugated
anti-Streptavidin antibody (FITC)适用: Streptomyces avidinii FACS, IF 宿主: 兔 Polyclonal FITC
anti-Streptavidin antibody (FITC)适用: Streptomyces avidinii FACS, FM, FLISA 宿主: 兔 Polyclonal FITC
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- 应用备注
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Immunohistochemistry Dilution: 1:1,500 - 1:5,000
Application Note: Anti-Streptavidin antibody has been tested by ELISA and dot blot and is suitable for western blot, and immunohistochemistry, as well as other assays requiring lot-to-lot consistency.
Western Blot Dilution: 1:3,000 - 1:12,000
ELISA Dilution: 1:200,000
Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- Streptavidin
- Abstract
- Streptavidin 产品
- 背景
- Background: Streptavidin antibody is a 60 kDa protein purified from the bacterium Streptomyces avidinii. Streptavidin homo-tetramers have an extraordinarily high affinity for biotin (also known as vitamin B7). With a dissociation constant (Kd) on the order of ~10-14 Mol/L,[1] the binding of biotin to streptavidin is one of the strongest non-covalent interactions known in nature. Streptavidin is used extensively in molecular biology and bio-nanotechnology due to the streptavidin-biotin complex's resistance to organic solvents, denaturants (e.g. guanidinium chloride), detergents (e.g. SDS, Triton), proteolytic enzymes, and extremes of temperature and pH . Anti-STREPTAVIDIN antibody is ideal for investigators involved in GFP and Epitope research.
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