DYKDDDDK Tag 抗体 (Agarose Beads)
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- 抗原 See all DYKDDDDK Tag products
- DYKDDDDK Tag
- 适用
- 请咨询
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This DYKDDDDK Tag antibody is conjugated to Agarose Beads
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应用范围
- Western Blotting (WB), Immunoprecipitation (IP)
- Supplier Product No.
- 200-350-383
- Supplier
- Rockland
- 原理
- DYKDDDDK Tag (Anti-FLAG®) Antibody Agarose Conjugated
- 序列
- DYKDDDDK
- 交叉反应 （详细）
- This product is intended for purification of proteins containing the FLAG® epitope tag sequence.
- 产品特性
- Synonyms: mouse anti-FLAG® tag agarose gel, mouse anti-DYKDDDDK agarose gel, Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys, D-Y-K-D-D-D-D-K, DDK, FLAG, FLAG antibody, anti-Flag, anti-DDK, DDK antibody
- 纯化方法
- DYKDDDDK Tag (Anti-FLAG®) Antibody Agarose Conjugated is a purified mouse IgG2a monoclonal antibody coupled to activated agarose.
- 免疫原
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Immunogen: DYKDDDDK Tag (Anti-FLAG®) Antibody Agarose Conjugated was produced in mice by repeated immunizations with a synthetic peptide corresponding to the FLAG® epitope tag peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH.
Immunogen Type: Conjugated Peptide
- 克隆位点
- 29E4-G7
- 亚型
- IgG2a kappa
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anti-DYKDDDDK Tag antibody
WB, ELISA, IP 宿主: 兔 Polyclonal unconjugated
anti-DYKDDDDK Tag antibodyWB, ELISA, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-DYKDDDDK Tag antibodyWB, ELISA, IP, DB, ISt 宿主: 小鼠 Monoclonal FG4R unconjugated
anti-DYKDDDDK Tag antibodyWB, ELISA, IP 宿主: 小鼠 Monoclonal 5A8E5 unconjugated
anti-DYKDDDDK Tag antibody适用: 标记 WB, ELISA, IP, IHC, FACS, ICC 宿主: 小鼠 Monoclonal 2F11G1 unconjugated
anti-DYKDDDDK Tag antibody适用: 人 WB 宿主: 兔 Polyclonal RB0493 unconjugated
anti-DYKDDDDK Tag antibody (HRP)WB, ELISA, IF 宿主: 小鼠 Monoclonal 5A8E5 HRP
anti-DYKDDDDK Tag antibodyWB, ELISA, IHC, FACS 宿主: 小鼠 Monoclonal 29E4-G7 unconjugated
anti-DYKDDDDK Tag antibodyWB, IP, IF, IC 宿主: 小鼠 Monoclonal unconjugated
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- 应用备注
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Application Note: DYKDDDDK Tag (Anti-FLAG®) Antibody Agarose Conjugated has been tested by IP and western blot and is optimally suited for immunoprecipitation and purification of FLAG® tagged fusion proteins.DYKDDDDK Tag (Anti-FLAG®) Antibody Agarose Conjugated recognizes the FLAG® epitope tag fused to either the amino- or carboxy-terminal ends or an internal location of targeted fusion proteins. The epitope tag peptide sequence was first derived from the 11-amino-acid leader peptide of the gene-10 product from bacteriophage T7. DYKDDDDK is the most commonly used hydrophilic octapeptide tag. Use D-Y-K-D-D-D-D-K peptide (p/n 000-000-383) for competitive elution to recover fusion protein (see protocol). Anti-FLAG® is a registered trademark of Sigma-Aldrich. Refer to the protocol for complete instructions for use including preferred buffers for elution. Do not use buffers that may denature the anti-DYKDDDDK antibody.
Immunoprecipitation Dilution: 10 μL resin binds >1 μg FLAG® fusion protein
- 限制
- 仅限研究用
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- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store vial at 4°C prior to opening.
- 有效期
- 12 months
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- 抗原
- DYKDDDDK Tag
- 别名
- FLAG (DYKDDDDK Tag 产品)
- 物质类
- Tag
- 背景
- Background: Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein's biochemical properties. Anti-epitope tag antibodies serve as universal detection reagents for any tag containing protein produced by recombinant means. Epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein.
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