TRPC6 抗体 (C-Term)
(1 reference)
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- 抗原 See all TRPC6 抗体
- TRPC6 (Transient Receptor Potential Cation Channel, Subfamily C, Member 6 (TRPC6))
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抗原表位
- C-Term
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适用
- 人, 小鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This TRPC6 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA
- Supplier Product No.
- 200-301-b59
- Supplier
- Rockland
- 原理
- TRPC6 Antibody
- 交叉反应 （详细）
- This antibody is specific for human TRPC6 protein.
- 产品特性
- Synonyms: mouse anti-TRPC6 Antibody, TRPC 6, TRP6, short transient receptor potential channel 6 and transient receptor potential cation channel subfamily C member 6
- 纯化方法
- This product was purified from concentrated tissue culture supernate by Protein A chromatography.
- 过滤
- Sterile filtered
- 免疫原
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Immunogen: This monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to a region near the carboxy terminus of human TRPC6 protein.
Immunogen Type: Conjugated Peptide
- 克隆位点
- 3F2-H10-F2
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product TRPC6 Primary Antibody
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anti-Transient Receptor Potential Cation Channel, Subfamily C, Member 6 (TRPC6) (AA 1-192) antibody
TRPC6 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Transient Receptor Potential Cation Channel, Subfamily C, Member 6 (TRPC6) (AA 500-600) antibodyTRPC6 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Transient Receptor Potential Cation Channel, Subfamily C, Member 6 (TRPC6) (AA 249-265), (Middle Region) antibodyTRPC6 适用: 人, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Transient Receptor Potential Cation Channel, Subfamily C, Member 6 (TRPC6) antibodyMicroarray verified TRPC6 适用: 人, 小鼠 IHC 宿主: 小鼠 Monoclonal MAB620777 unconjugated
anti-Transient Receptor Potential Cation Channel, Subfamily C, Member 6 (TRPC6) (AA 24-38), (Intracellular), (N-Term) antibodyTRPC6 适用: 小鼠, 大鼠 WB, IHC, IF 宿主: 豚鼠 Polyclonal unconjugated
anti-Transient Receptor Potential Cation Channel, Subfamily C, Member 6 (TRPC6) antibodyMicroarray verified TRPC6 适用: 人, 小鼠 IHC 宿主: 小鼠 Monoclonal MAB591644 unconjugated
anti-Transient Receptor Potential Cation Channel, Subfamily C, Member 6 (TRPC6) antibodyMicroarray verified TRPC6 适用: 人, 小鼠 IHC 宿主: 小鼠 Monoclonal MAB447436 unconjugated
anti-Transient Receptor Potential Cation Channel, Subfamily C, Member 6 (TRPC6) (C-Term) antibodyVerified TRPC6 适用: 人 WB, ELISA, FACS 宿主: 山羊 Polyclonal unconjugated
anti-Transient Receptor Potential Cation Channel, Subfamily C, Member 6 (TRPC6) (AA 732-931) antibodyTRPC6 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Transient Receptor Potential Cation Channel, Subfamily C, Member 6 (TRPC6) (C-Term) antibodyTRPC6 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Immunohistochemistry Dilution: 2.5 μg/mL
Application Note: Anti-TRPC6 monoclonal antibody (200-301-B59) clone # 3F2.H10.F2 was developed by Rockland Immunochemicals Inc. against human TRPC6 using conventional hybridoma technology by fusing splenocytes of a host animal immunized with a synthetic peptide corresponding to the cytosolic domain of TRPC6 with myeloma cells. The screening of clones during the subcloning process was based on immunohistochemistry using human tissue microarrays. The pathologist analyzing the staining patterns of clones reported that the antibody shows strong to moderate staining consistent with the localization of human TRPC6 in adrenal cortex, neurons, Purkinje cells, colon epithelium, cardiac myocytes, renal tubules, hepatocytes, skeletal muscle, pancreatic exocrine and islet cells, germinal center lymphocytes, plasma cells, Sertoli cells of the testes as well as staining more faintly other tissues known to be positive for the target protein (e.g., respiratory epithelium). Prostate and placenta were negative for staining. The antibody produced minimal to no background staining and appeared very specific at 2.5 μg/mL. The pattern of reactivity observed for this clone was also similar to other antibodies used for benchmarking purposes. Specific conditions for reactivity should be optimized by the end user, however, we suggest the use of formalin-fixed paraffin-embedded sections for immunohistochemistry. No pre-treatment of sample is required. While immunohistochemistry was used as the primary screening and release validation immunoassay, clone #3F2.H10.F2 was also screened by western blotting against known positive and negative control lysates. A single band is detected by this antibody in TRPC6 positive cells and tissues, however, the molecular weight of the band (~30 kDa) is not consistent with full length human TRPC6 (181 kDa). The band detected by this antibody may be the cleaved cytosolic domain of TRPC6 as the immunogen used for antibody production corresponds to an amino acid sequence located within this domain. However, no additional data is available to elucidate the molecular composition of this band.
Western Blot Dilution: 1:500- 1:2,000
ELISA Dilution: 1:10,000 - 1:50,000
Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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: "Human Digital Meissner Corpuscles Display Immunoreactivity for the Multifunctional Ion Channels Trpc6 and Trpv4." in: Anatomical record (Hoboken, N.J. : 2007), Vol. 300, Issue 6, pp. 1022-1031, (2018) (PubMed).
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: "Human Digital Meissner Corpuscles Display Immunoreactivity for the Multifunctional Ion Channels Trpc6 and Trpv4." in: Anatomical record (Hoboken, N.J. : 2007), Vol. 300, Issue 6, pp. 1022-1031, (2018) (PubMed).
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- 抗原
- TRPC6 (Transient Receptor Potential Cation Channel, Subfamily C, Member 6 (TRPC6))
- 别名
- TRPC6 (TRPC6 产品)
- 背景
- Background: TRPC6, also known as TRP6, short transient receptor potential channel 6 and transient receptor potential cation channel subfamily C member 6, is thought to form a receptor-activated non-selective calcium permeant cation channel. TRPC6 is probably operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. It is activated by diacylglycerol (DAG) in a membrane-delimited fashion, independently of protein kinase C and may not to be activated by intracellular calcium store depletion. Defects in this gene are a cause of focal segmental glomerulosclerosis (FSGS). Expression of this protein has been reported in tissues such as placenta, lung, spleen, ovary, small intestine, and renal podocytes. Immunohistochemistry studies using polyclonal antibodies to this target have shown moderate to strong staining in cell types such as neurons, breast, respiratory, squamous and prostate epithelium, epidermis, placental trophoblasts, dendritic cells, and subsets of immune cells, and faint to moderate staining of adrenal, colon, ganglion cells, hepatocytes, heart, and testis.
- 基因ID
- 7225, 5730102
- UniProt
- Q9Y210
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