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PDCD4 抗体 (pSer457)

Name: PDCD4 抗体 (pSer457)
SKU: ABIN964683
Availability: OutOfStock

Rockland 200-301-964 PDCD4 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 小鼠 Monoclonal 9G6 unconjugated

Western blot using Protein A purified Mouse Monoclonal anti-Pdcd4 pS457 antibody shows detection of phosphorylated Pdcd4 (indicated by arrowhead at ~62 kDa) in NIH-3T3 cells after 5 min treatment with 30 ng/mL PDGF (lane 2). (PDCD4 抗体 (pSer457))

Antibody 200-301-964 has been tested in immunohistochemistry, analyzed by an anatomic pathologist and validated for use in IHC applications against formalin-fixed, paraffin-embedded human tissues. (PDCD4 抗体 (pSer457))

IHC

Western blot using Immunochemicals Protein A purified Mouse Monoclonal anti-Pdcd4 pS457 antibody against recombinant PDCD4 protein. (PDCD4 抗体 (pSer457))

3 images

(1 reference)

产品编号 ABIN964683

Supplier Product No.: 200-301-964

发货至: 中国

Datasheet as PDF

抗原 See all PDCD4 抗体

PDCD4 (Programmed Cell Death 4 (PDCD4))
抗原表位
15

8

7

7

7

5

5

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1
pSer457
适用
96

62

51

6

5

4

4

3

3

3

3

3

3

1

1
人, 小鼠
宿主
90

4

2

1
小鼠
Compatible Secondaries
克隆类型
86

11
单克隆
标记
58

4

3

3

3

3

3

3

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1
This PDCD4 antibody is un-conjugated
应用范围
73

41

29

14

14

14

12

9

6

3

3

2

1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Supplier Product No.

200-301-964

Supplier

Rockland

原理

Pdcd4 phospho S457 Antibody

交叉反应（详细）

This antibody is specific for human Pdcd4 protein phosphorylated at Ser457.

产品特性

Synonyms: mouse anti-Pdcd4 pS457 Antibody, Death up-regulated gene protein antibody, Dug antibody, H731 antibody, Ma3 antibody, Neoplastic transformation inhibitor antibody, Neoplastic transformation inhibitor protein antibody, Nuclear antigen H731 antibody

纯化方法

Pdcd4 phospho S457 Antibody was purified from concentrated tissue culture supernate by Protein A chromatography.

过滤

Sterile filtered

免疫原

Immunogen: This monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding Ser457 of the human Pdcd4 protein.
Immunogen Type: Conjugated Peptide

克隆位点

9G6

亚型

IgG1 kappa

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PDCD4 适用: 人 WB, ELISA, IHC, IP 宿主: 兔 Polyclonal unconjugated

应用备注

Immunohistochemistry Dilution: 1:1,000 - 1:5,000
Application Note: Pdcd4 phospho S457 Antibody is tested by ELISA, immunohistochemistry, and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 62 kDa in size corresponding to phosphorylated Pdcd4 protein by western blotting in the appropriate cell lysate or extract. This phospho-specific monoclonal antibody reacts with human Pdcd4 pS457 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.
Western Blot Dilution: 1:2,000 - 1:10,000
ELISA Dilution: 1:20,000 - 1:100,000
Other: User Optimized

限制

仅限研究用
状态

Liquid

浓度

1.0 mg/mL

缓冲液

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

有效期

12 months
Huang, Wu, Loeb, Hsu, Heidersbach, Brincat, Horiuchi, Lebbink, Mo, Goga, McManus: "Up-regulation of miR-21 by HER2/neu signaling promotes cell invasion." in: The Journal of biological chemistry, Vol. 284, Issue 27, pp. 18515-24, (2009) (PubMed).
抗原

PDCD4 (Programmed Cell Death 4 (PDCD4))

别名

PDCD4 (PDCD4 产品)

背景

Background: Programmed cell death 4 (Pdcd4) is a novel tumor suppressor. Pdcd4 directly inhibits the helicase activity of eukaryotic translation initiation factor 4A (eIF4A), a component of the translation initiation complex. Pdcd4 also suppresses the transactivation of activator protein-1 (AP-1)-responsive promoters by c-Jun. Pdcd4 contains two Akt phosphorylation sites, one at Ser67 and the other at Ser457. The phosphorylation of Pdcd4 by Akt causes nuclear translocation of Pdcd4 and a significant decrease in the ability of Pdcd4 to interfere with the transactivation of AP-1-responsive promoters by c-Jun.

基因ID

27250, 21735596

UniProt

Q53EL6