Luciferase 抗体
(2 references)
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- 抗原 See all Luciferase 抗体
- Luciferase
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适用
- Photinus pyralis
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This Luciferase antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Fluorescence Microscopy (FM)
- Supplier Product No.
- 200-101-c44
- Supplier
- Rockland
- 原理
- Luciferase Antibody
- 交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Luciferase [Photinus pyralis (Firefly)].
- 产品特性
- Synonyms: goat anti-Luciferase Antibody, Luciferin 4-monooxygenase, Photinus pyralis (Common eastern firefly) (Lampyris pyralis)
- 纯化方法
- This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
- 过滤
- Sterile filtered
- 免疫原
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Immunogen: Luciferase [Photinus pyralis (Firefly)]
Immunogen Type: Native Protein
- 亚型
- IgG
- Top Product
- Discover our top product Luciferase Primary Antibody
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anti-Luciferase antibody
适用: Firefly ELISA, IF (p), IF (cc), IHC (fro), IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Luciferase antibody适用: Firefly WB, IF 宿主: 小鼠 Monoclonal 2C2 unconjugated
anti-Luciferase antibody适用: Firefly WB, ELISA, IHC 宿主: 小鼠 Monoclonal unconjugated
anti-Luciferase antibody适用: Photinus pyralis WB 宿主: 小鼠 Monoclonal Luci17 unconjugated
anti-Luciferase (C-Term) antibody适用: Photinus pyralis WB 宿主: 山羊 Polyclonal unconjugated
anti-Luciferase (N-Term) antibody适用: Photinus pyralis WB, IHC 宿主: 小鼠 Monoclonal Luci17 unconjugated
anti-Luciferase antibody适用: Photinus pyralis WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Luciferase antibody适用: Photinus pyralis WB, IP 宿主: 小鼠 Monoclonal 2D4 unconjugated
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- 应用备注
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Immunohistochemistry Dilution: 1:1500
Application Note: Anti-Luciferase Antibody has been tested in Western Blot, IHC, IF, and ELISA. Expect a band ~60 kDa in appropriate cell lysates. Although not tested, this antibody would be useful in immunoprecipitation, immunocytochemistry, and most immunological methods requiring high titers and specificity.
Western Blot Dilution: 1:1000 - 1:5000
ELISA Dilution: 1:200 - 1:1000
IF Microscopy Dilution: User Optimized
Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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: "Unresolved endoplasmic reticulum stress engenders immune-resistant, latent pancreatic cancer metastases." in: Science (New York, N.Y.), Vol. 360, Issue 6394, (2018) (PubMed).
: "Increased expression of the urokinase plasminogen activator system by Helicobacter pylori in gastric epithelial cells." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 295, Issue 3, pp. G431-41, (2008) (PubMed).
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: "Unresolved endoplasmic reticulum stress engenders immune-resistant, latent pancreatic cancer metastases." in: Science (New York, N.Y.), Vol. 360, Issue 6394, (2018) (PubMed).
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- 抗原
- Luciferase
- Abstract
- Luciferase 产品
- 背景
- Background: Anti luciferase Antibody recognizes luciferase that is commonly used in biological research as a reporter to assess the transcriptional activity in cells that are transfected with a genetic construct containing the luciferase gene under the control of a promoter of interest. Luciferase can also be used to detect the level of cellular ATP in cell viability assays or for kinase activity assays. Additionally proluminescent molecules that are converted to luciferin upon activity of a particular enzyme can be used to detect enzyme activity in coupled or two-step luciferase assays. Such substrates have been used to detect caspase activity and cytochrome P450 activity, among others.
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