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ZNF148 抗体

Rockland 100-401-685 ZNF148 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
Rockland
产品编号 ABIN964672
Supplier Product No.: 100-401-685
发货至: 中国
  • 抗原 See all ZNF148 抗体
    ZNF148 (Zinc Finger Protein 148 (ZNF148))
    适用
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    宿主
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    克隆类型
    • 38
    多克隆
    标记
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    This ZNF148 antibody is un-conjugated
    应用范围
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    Western Blotting (WB), ELISA
    Supplier Product No.
    100-401-685
    Supplier
    Rockland
    原理
    ZBP-89 Antibody
    交叉反应 (详细)
    This polyclonal antibody is specific for human ZBP-89.
    产品特性
    Synonyms: rabbit anti-ZBP-89 Antibody, Transcription factor ZBP89 antibody, Zinc finger DNA binding protein 89 antibody, Zinc finger protein 148 antibody, ZNF 148 antibody, Zinc finger protein 148, Transcription factor ZBP-89, ZNF148
    纯化方法
    Anti-ZBP89 antibody was prepared from monospecific antiserum by delipidation and defibrination.
    过滤
    Sterile filtered
    免疫原

    Immunogen: Purified full length ZBP-89 recombinant protein expressed in E.coli.

    Immunogen Type: Recombinant Protein

    Top Product
    Discover our top product ZNF148 Primary Antibody
  • 应用备注

    Application Note: This polyclonal antibody reacts with human ZBP-89 in a variety of tested immunological assays including western blot and ELISA.  Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation. For immunoblotting a 1:5,000 dilution is recommended. A band at approximately 89 kDa corresponding to human ZBP-89 is detected. Human monocytes or macrophages or nuclear extracts from PMA treated U937 cells can be used as a positive control. For ELISA a 1:10,000 to 1:30,000 dilution is recommended. Researchers should determine optimal titers for other applications.

    Western Blot Dilution: 1:5,000

    ELISA Dilution: 1:10,000 - 1:30,000

    Other: User Optimized

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    90 mg/mL
    缓冲液

    Buffer: None

    Stabilizer: None

    Preservative: 0.1 % (w/v) Sodium Azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Store ZBP89 Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    有效期
    12 months
  • Ohneda, Ohmori, Ishijima, Nakano, Yamamoto: "Characterization of a functional ZBP-89 binding site that mediates Gata1 gene expression during hematopoietic development." in: The Journal of biological chemistry, Vol. 284, Issue 44, pp. 30187-99, (2009) (PubMed).

    Guyot, Murai, Fujiwara, Valverde-Garduno, Hammett, Wells, Dear, Orkin, Porcher, Vyas: "Characterization of a megakaryocyte-specific enhancer of the key hemopoietic transcription factor GATA1." in: The Journal of biological chemistry, Vol. 281, Issue 19, pp. 13733-42, (2006) (PubMed).

  • 抗原
    ZNF148 (Zinc Finger Protein 148 (ZNF148))
    别名
    ZNF148 (ZNF148 产品)
    背景
    Background: The GI tract abundantly expresses growth factors many of which bind and activate the EGF receptor present on mucosal cells. One such factor is the zinc finger protein (ZBP-89) that binds to a GC-rich DNA element in the gastrin promoter and confers EGF responsiveness. The full-length protein functions as a repressor of growth factor signals regulating the gastrin promoter. Several other growth related promoters are also regulated by ZBP-89. ZBP-89 is one of a family of related transcriptional regulators. It has been reported in recent studies that ZBP-89 regulates growth in part by stimulating the cyclin-dependent kinase inhibitor, p21waf1, in a butyrate-dependent manner through recruitment of the histone acetyl transferase p300. Moreover, ZBP-89 triggers growth arrest in a p53-dependent manner by preventing nuclear export of p53. ZBP-89 also induces apoptosis, but this process occurs independent of p53.
    基因ID
    256711
    UniProt
    Q9UQR1
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