Fluorescein 抗体 (Alkaline Phosphatase (AP))
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- 抗原
- Fluorescein
- 片段
- F(ab')2 fragment
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- Alkaline Phosphatase (AP)
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
- Supplier Product No.
- 700-105-096
- Supplier
- Rockland
- 原理
- F(Ab')2 Fluorescein Antibody Alkaline Phosphatase Conjugated
- 交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Goat Serum and Fluorescein conjugated Bovine Serum Albumin.
- 产品特性
- Synonyms: goat F(ab')2 anti-Fluorescein Antibody alkaline phosphatase Conjugation, goat F(ab')2 anti-Fluorescein alk phos Conjugated Antibody, goat F(ab')2 anti-FITC Antibody alkaline phosphatase Conjugation
- 纯化方法
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using Fluorescein IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
- 过滤
- Sterile filtered
- 免疫原
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Immunogen: Fluorescein conjugated to Goat IgG
Immunogen Type: Other
- 亚型
- IgG
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anti-Fluorescein antibody
WB, ELISA, IHC, FACS, IF 宿主: 小鼠 Monoclonal 8B9-C6-D3 unconjugated
anti-Fluorescein antibodyWB, ELISA, IHC, FACS, IF 宿主: 小鼠 Monoclonal 8B9-C6-D3 unconjugated
anti-Fluorescein antibodyWB, ELISA, IHC, FACS 宿主: 山羊 Polyclonal unconjugated
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- 应用备注
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Immunohistochemistry Dilution: 1:200 - 1:1,000
Application Note: F(ab')2 Anti-Fluorescein Antibody Conjugated to Alkaline Phosphatase has been tested by ELISA and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Western Blot Dilution: 1:500 - 1:2,500
ELISA Dilution: 1:38,000
Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.1 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
- 有效期
- 12 months
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- 抗原
- Fluorescein
- 物质类
- Chemical
- 背景
- Background: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
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