YBX3/DBPA 抗体
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- 抗原 See all YBX3/DBPA (YBX3) products
- YBX3/DBPA (YBX3) (Y Box Binding Protein 3 (YBX3))
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适用
- Borrelia burgdorferi
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This YBX3/DBPA antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA
- Supplier Product No.
- 200-401-b98
- Supplier
- Rockland
- 原理
- DbpA Antibody
- 交叉反应 （详细）
- This antibody is specific for Borrelia burgdorferi DbpA protein.
- 产品特性
- Synonyms: rabbit anti-DbpA Antibody, Decorin-binding Protein, Borrelia burgdorferi DbpA, dbp-A, dbp A
- 纯化方法
- This product was Protein-A purified and cross-adsorbed against MBP from monospecific antiserum by chromatography.
- 免疫原
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Immunogen: MBP-fusion protein corresponding to Borrelia burgdorferi Dbp-A protein.
Immunogen Type: Recombinant Protein
- 亚型
- IgG
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anti-Y Box Binding Protein 3 (YBX3) (AA 201-300) antibody
YBX3 适用: 小鼠 WB, IF (cc), IF (p), ELISA, IHC (fro), IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Application Note: This protein-A purified antibody has been tested for use in ELISA and Western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 18.5 kDa in size corresponding to Borrelia burgdorferi DbpA protein by Western blotting in the appropriate cell lysate or extract.
Western Blot Dilution: 1:1,000
ELISA Dilution: 1:5,000
Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- YBX3/DBPA (YBX3) (Y Box Binding Protein 3 (YBX3))
- 别名
- DBP A (YBX3 产品)
- 背景
- Background: This product is antibody made against DbpA, or Decorin Binding Protein A from the spirochete Borrelia burgdorferi, which is carried by Ixodes ticks. DbpA from other microbial organisms such as E. coli (ATP-dependent RNA helicase DbpA) are significantly different. The spirochete migrates from the tick midgut during tick feeding to tick salivary glands and are thus transmitted to the mammal host. This transition may be facilitated by changes in expression of some B. burgdorferi genes. Spirochetal surface adhesions mediate attachment to decorin, a major component of the host extracellular matrix, enabling bacteria to colonize in mammalian tissues. It is believed that expression of the various proteins associated with the spirochete may be regulated by the changes in tick life cycle, changes in conditions during tick feeding (such as temperature, pH , and nutrients) and/or in coordination with the course of infection of the mammal host.
- 基因ID
- 1194347
- NCBI登录号
- WP_010890380
- UniProt
- O50917
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