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Biotin 抗体

Rockland 600-401-098 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
Rockland
产品编号 ABIN964580
Supplier Product No.: 600-401-098
发货至: 中国
  • 抗原 See all Biotin products
    Biotin
    适用
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    宿主
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    克隆类型
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    多克隆
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    This Biotin antibody is un-conjugated
    应用范围
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    Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
    Supplier Product No.
    600-401-098
    Supplier
    Rockland
    原理
    Biotin Antibody
    交叉反应 (详细)
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Biotin conjugated IgG and Biotin conjugated Bovine Serum Albumin.
    产品特性
    Synonyms: rabbit anti Biotin Antibody, rabbit anti-biotin Antibody
    纯化方法
    Biotin Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Biotin coupled to sepharose beads.
    过滤
    Sterile filtered
    免疫原

    Immunogen: Biotin conjugated to Keyhole Limpet Hemocyanin (KLH)

    Immunogen Type: Other

    亚型
    IgG
  • 应用备注

    Immunohistochemistry Dilution: User Optimized

    Application Note: Anti-Biotin Antibody has been tested by ELISA and is suitable for immunoprecipitation, immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.

    Western Blot Dilution: 1:2,000 - 1:10,000

    ELISA Dilution: 1:200,000

    Other: User Optimized

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    1.02 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    This product is stable for several weeks at 4° C as an undiluted liquid. Aliquot contents and freeze at -20° C or below for extended storage.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use. 
    有效期
    12 months
  • Goulielmaki, Ioannidou, Tsekrekou, Stratigi, Poutakidou, Gkirtzimanaki, Aivaliotis, Evangelou, Topalis, Altmüller, Gorgoulis, Chatzinikolaou, Garinis: "Tissue-infiltrating macrophages mediate an exosome-based metabolic reprogramming upon DNA damage." in: Nature communications, Vol. 11, Issue 1, pp. 42, (2020) (PubMed).

  • 抗原
    Biotin
    Abstract
    Biotin 产品
    物质类
    Chemical
    背景
    Background: Biotin Antibody detects Biotin. Biotin is a water-soluble B-complex vitamin (vitamin B7). It is composed of a ureido (tetrahydroimidizalone) ring fused with a tetrahydrothiophene ring. A valeric acid substituent is attached to one of the carbon atoms of the tetrahydrothiophene ring. Biotin is a coenzyme for carboxylase enzymes, involved in the synthesis of fatty acids, isoleucine, and valine, and in gluconeogenesis. Biotin is necessary for cell growth, the production of fatty acids, and the metabolism of fats and amino acids. Anti-Biotin Antibody is ideal for investigators involved in Cell Signaling and Cell Biology research.
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