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ATG13 抗体 (pSer318)

Rockland 600-401-c49 ATG13 适用: 人 WB, ELISA, DB, FACS 宿主: 兔 Polyclonal unconjugated
Rockland
产品编号 ABIN964569
Supplier Product No.: 600-401-c49
发货至: 中国
  • 抗原 See all ATG13 抗体
    ATG13 (Autophagy Related 13 (ATG13))
    抗原表位
    • 15
    • 10
    • 7
    • 7
    • 5
    • 5
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    pSer318
    适用
    • 51
    • 31
    • 30
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    宿主
    • 66
    • 3
    克隆类型
    • 65
    • 4
    多克隆
    标记
    • 26
    • 7
    • 7
    • 6
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This ATG13 antibody is un-conjugated
    应用范围
    • 46
    • 33
    • 14
    • 13
    • 13
    • 9
    • 7
    • 5
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    Western Blotting (WB), ELISA, Dot Blot (DB), Flow Cytometry (FACS)
    Supplier Product No.
    600-401-c49
    Supplier
    Rockland
    原理
    ATG13 phospho S318 Antibody
    交叉反应 (详细)
    This affinity-purified antibody is directed against the phosphorylated form of human ATG13 protein at the pS318 residue.
    产品特性
    Synonyms: rabbit anti-ATG13 pS318 Antibody, ATG-13, ATG 13, Autophagy-related protein 13, KIAA0652
    纯化方法
    The product was affinity purified from monospecific antiserum by immunoaffinity purification.
    过滤
    Sterile filtered
    免疫原

    Immunogen: Anti-ATG13 pS318 antibody was prepared by repeated immunizations with a synthetic peptide corresponding to the region near S318 of ATG13.

    Immunogen Type: Conjugated Peptide

    亚型
    IgG
    Top Product
    Discover our top product ATG13 Primary Antibody
  • 应用备注

    Flow Cytometry Dilution: User Optimized

    Application Note: This affinity purified antibody has been tested for use in ELISA and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 56.6 kDa in size corresponding to human phosphorylated ATG13 protein by western blotting in the appropriate stimulated tissue or cell lysate or extract. 

    Western Blot Dilution: 1:2000

    ELISA Dilution: 1:25,000-1:175,000

    Other: User Optimized

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    1.0 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    有效期
    12 months
  • Jia, Bonifacino: "Negative regulation of autophagy by UBA6-BIRC6-mediated ubiquitination of LC3." in: eLife, Vol. 8, (2020) (PubMed).

    Izumi, Li, Shibaki, Mori, Yasunami, Sato, Matsunaga, Mae, Kodama, Kamijo, Kaneko, Nakagawara: "Recycling endosomal CD133 functions as an inhibitor of autophagy at the pericentrosomal region." in: Scientific reports, Vol. 9, Issue 1, pp. 2236, (2019) (PubMed).

    Wang, Maxwell, Joo, Gwon, Messing, Mishra, Shaw, Ward, Quan, Sakurada, Pruett-Miller, Bertorini, Vogel, Kim, Peng, Taylor, Kundu: "ULK1 and ULK2 Regulate Stress Granule Disassembly Through Phosphorylation and Activation of VCP/p97." in: Molecular cell, Vol. 74, Issue 4, pp. 742-757.e8, (2019) (PubMed).

    Wallot-Hieke, Verma, Schlütermann, Berleth, Deitersen, Böhler, Stuhldreier, Wu, Seggewiß, Peter, Gohlke, Mizushima, Stork: "Systematic analysis of ATG13 domain requirements for autophagy induction." in: Autophagy, Vol. 14, Issue 5, pp. 743-763, (2019) (PubMed).

    Thayer, Awad, Hegdekar, Sarkar, Tesfay, Burt, Zeng, Feldman, Lipinski: "The PARK10 gene USP24 is a negative regulator of autophagy and ULK1 protein stability." in: Autophagy, pp. 1-14, (2019) (PubMed).

    Fuqua, Mere, Kronemberger, Blomme, Bae, Turner, Harris, Scudese, Edwards, Ebert, de Sousa, Bodine, Yang, Adams, Lira: "ULK2 is essential for degradation of ubiquitinated protein aggregates and homeostasis in skeletal muscle." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 33, Issue 11, pp. 11735-11745, (2019) (PubMed).

    Jeong, Simoneschi, Keegan, Melville, Adler, Saraf, Florens, Washburn, Cavasotto, Fenyö, Cuervo, Rossi, Pagano: "The ULK1-FBXW5-SEC23B nexus controls autophagy." in: eLife, Vol. 7, (2019) (PubMed).

    Wang, Wang, Zhang, Luo, Liu, Xu, Diao, Liao, Liu: "Phosphorylation of ULK1 affects autophagosome fusion and links chaperone-mediated autophagy to macroautophagy." in: Nature communications, Vol. 9, Issue 1, pp. 3492, (2018) (PubMed).

    Baron, Boudi, Dias, Schilling, Nölle, Vizcay-Barrena, Rattray, Jungbluth, Scheper, Fleck, Bates, Fanto: "Stall in Canonical Autophagy-Lysosome Pathways Prompts Nucleophagy-Based Nuclear Breakdown in Neurodegeneration." in: Current biology : CB, Vol. 27, Issue 23, pp. 3626-3642.e6, (2018) (PubMed).

    Jung, Nayak, Schaeffer, Starzetz, Kirsch, Müller, Dikic, Mittelbronn, Behrends: "Multiplex image-based autophagy RNAi screening identifies SMCR8 as ULK1 kinase activity and gene expression regulator." in: eLife, Vol. 6, (2017) (PubMed).

    Hieke, Löffler, Kaizuka, Berleth, Böhler, Drießen, Stuhldreier, Friesen, Assani, Schmitz, Peter, Diedrich, Dengjel, Holland, Simonsen, Wesselborg, Mizushima, Stork: "Expression of a ULK1/2 binding-deficient ATG13 variant can partially restore autophagic activity in ATG13-deficient cells." in: Autophagy, Vol. 11, Issue 9, pp. 1471-83, (2016) (PubMed).

    Puente, Hendrickson, Jiang: "Nutrient-regulated Phosphorylation of ATG13 Inhibits Starvation-induced Autophagy." in: The Journal of biological chemistry, Vol. 291, Issue 11, pp. 6026-35, (2016) (PubMed).

    Park, Jung, Seo, Otto, Grunwald, Kim, Moriarity, Kim, Starker, Nho, Voytas, Kim: "The ULK1 complex mediates MTORC1 signaling to the autophagy initiation machinery via binding and phosphorylating ATG14." in: Autophagy, Vol. 12, Issue 3, pp. 547-64, (2016) (PubMed).

    Jiao, Su, Dong, Zhang, Xie, Yao, Chen, Wang, Liou, You: "Chaperone-like protein p32 regulates ULK1 stability and autophagy." in: Cell death and differentiation, Vol. 22, Issue 11, pp. 1812-23, (2016) (PubMed).

    Rosenberg, Lafitte, Grant, Chen, Cleveland, Duckett: "Development of an HTS-Compatible Assay for the Discovery of Ulk1 Inhibitors." in: Journal of biomolecular screening, Vol. 20, Issue 7, pp. 913-20, (2016) (PubMed).

    Egan, Chun, Vamos, Zou, Rong, Miller, Lou, Raveendra-Panickar, Yang, Sheffler, Teriete, Asara, Turk, Cosford, Shaw: "Small Molecule Inhibition of the Autophagy Kinase ULK1 and Identification of ULK1 Substrates." in: Molecular cell, Vol. 59, Issue 2, pp. 285-97, (2015) (PubMed).

    Puustinen, Rytter, Mortensen, Kohonen, Moreira, Jäättelä: "CIP2A oncoprotein controls cell growth and autophagy through mTORC1 activation." in: The Journal of cell biology, Vol. 204, Issue 5, pp. 713-27, (2014) (PubMed).

    Stanton, Dutta, Zhang, Polavaram, Leontovich, Hönscheid, Sinicrope, Tindall, Muders, Datta: "Autophagy control by the VEGF-C/NRP-2 axis in cancer and its implication for treatment resistance." in: Cancer research, Vol. 73, Issue 1, pp. 160-71, (2013) (PubMed).

    Nazio, Strappazzon, Antonioli, Bielli, Cianfanelli, Bordi, Gretzmeier, Dengjel, Piacentini, Fimia, Cecconi: "mTOR inhibits autophagy by controlling ULK1 ubiquitylation, self-association and function through AMBRA1 and TRAF6." in: Nature cell biology, Vol. 15, Issue 4, pp. 406-16, (2013) (PubMed).

    Joo, Dorsey, Joshi, Hennessy-Walters, Rose, McCastlain, Zhang, Iyengar, Jung, Suen, Steeves, Yang, Prater, Kim, Thompson, Youle, Ney, Cleveland, Kundu: "Hsp90-Cdc37 chaperone complex regulates Ulk1- and Atg13-mediated mitophagy." in: Molecular cell, Vol. 43, Issue 4, pp. 572-85, (2011) (PubMed).

  • 抗原
    ATG13 (Autophagy Related 13 (ATG13))
    别名
    ATG13 (ATG13 产品)
    背景
    Background: ATG13 is a target of the TOR kinase signaling pathway that regulates autophagy through the control of the phosphorylation status of ATG13 and ULK1 through their stable complex, and the regulation of ATG13-ULK1-RB1CC1. ATG13 also forms a stable complex with FIP200. Ulk1 phosphorylates ATG13 on S318 and promotes its release to damaged mitochondria. Autophagy is a normal process in eukaryotes required for turnover of cellular components during starvation and stress. It plays an essential role in cellular differentiation, cell death and aging. Defects in this evolutionarily conserved process may contribute to certain human diseases such as cancer, neurodegenerative diseases, muscular disorders and pathogen infections. ATG13 is one of several ATG genes required for autophagosome formation in mammalian cells. mTOR interacts with this complex in a nutrient dependent manner and phosphorylates Atg13 and ULK1.
    基因ID
    9776
    NCBI登录号
    NP_001136145
    UniProt
    O75143
    途径
    PI3K-Akt Signaling, Autophagy
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