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ATG13 抗体

Name: ATG13 抗体
SKU: ABIN964567
Availability: OutOfStock

Rockland 600-401-c50 ATG13 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated

Western blot using affinity purified anti-ATG13 antibody shows detection of ATG13 in 293T cells engineered to coexpress Ulk1 and Atg13 (Ulk1 + Atg13), right lane, but not in the left lane in which was loaded kinase-dead hypophosphorylated Ulk1-K46A mutant + ATG13. (ATG13 抗体)

1 image

(3 references)

产品编号 ABIN964567

Supplier Product No.: 600-401-c50

发货至: 中国

Datasheet as PDF

抗原 See all ATG13 抗体

ATG13 (Autophagy Related 13 (ATG13))
适用
52

31

30

3

3

3

3

3

2

2

2

1

1
人
宿主
67

3
兔
Compatible Secondaries
克隆类型
66

4
多克隆
标记
27

7

7

6

3

3

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This ATG13 antibody is un-conjugated
应用范围
47

34

14

13

13

9

8

5

3

3

3

3

2

1

1
Western Blotting (WB), ELISA

Supplier Product No.

600-401-c50

Supplier

Rockland

原理

ATG13 Antibody

交叉反应（详细）

This affinity-purified antibody is directed against human ATG13 protein.

产品特性

Synonyms: rabbit anti-ATG13 Antibody, ATG-13, ATG 13, Autophagy-related protein 13, KIAA0652

纯化方法

The product was affinity purified from monospecific antiserum by immunoaffinity purification.

过滤

Sterile filtered

免疫原

Immunogen: This affinity purified antibody was prepared by repeated immunizations with a synthetic peptide corresponding to the S318 region of ATG13.
Immunogen Type: Conjugated Peptide

亚型

IgG

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ATG13 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

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ATG13 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-Autophagy Related 13 (ATG13) (pSer318) antibody
ATG13 适用: 人 WB, ELISA, DB, FACS 宿主: 兔 Polyclonal unconjugated

anti-Autophagy Related 13 (ATG13) (AA 1-30) antibody
ATG13 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-Autophagy Related 13 (ATG13) antibody (HRP)
ATG13 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal HRP

anti-Autophagy Related 13 (ATG13) antibody (Biotin)
ATG13 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal Biotin

应用备注

Application Note: This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 56.6 kDa in size corresponding to human ATG13 protein by western blotting in the appropriate stimulated tissue or cell lysate or extract.
Western Blot Dilution: 1:1000
ELISA Dilution: 1:220,000-1:250,000
Other: User Optimized

限制

仅限研究用
状态

Liquid

浓度

1.24 mg/mL

缓冲液

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

有效期

12 months
Stanton, Dutta, Zhang, Polavaram, Leontovich, Hönscheid, Sinicrope, Tindall, Muders, Datta: "Autophagy control by the VEGF-C/NRP-2 axis in cancer and its implication for treatment resistance." in: Cancer research, Vol. 73, Issue 1, pp. 160-71, (2013) (PubMed).

Nazio, Strappazzon, Antonioli, Bielli, Cianfanelli, Bordi, Gretzmeier, Dengjel, Piacentini, Fimia, Cecconi: "mTOR inhibits autophagy by controlling ULK1 ubiquitylation, self-association and function through AMBRA1 and TRAF6." in: Nature cell biology, Vol. 15, Issue 4, pp. 406-16, (2013) (PubMed).

Joo, Dorsey, Joshi, Hennessy-Walters, Rose, McCastlain, Zhang, Iyengar, Jung, Suen, Steeves, Yang, Prater, Kim, Thompson, Youle, Ney, Cleveland, Kundu: "Hsp90-Cdc37 chaperone complex regulates Ulk1- and Atg13-mediated mitophagy." in: Molecular cell, Vol. 43, Issue 4, pp. 572-85, (2011) (PubMed).
抗原

ATG13 (Autophagy Related 13 (ATG13))

别名

ATG13 (ATG13 产品)

背景

Background: ATG13 is a target of the TOR kinase signaling pathway that regulates autophagy through the control of the phosphorylation status of ATG13 and ULK1 through their stable complex, and the regulation of ATG13-ULK1-RB1CC1. ATG13 also forms a stable complex with FIP200. Ulk1 phosphorylates ATG13 on S318 and promotes its release to damaged mitochondria. Autophagy is a normal process in eukaryotes required for turnover of cellular components during starvation and stress. It plays an essential role in cellular differentiation, cell death and aging. Defects in this evolutionarily conserved process may contribute to certain human diseases such as cancer, neurodegenerative diseases, muscular disorders and pathogen infections. ATG13 is one of several ATG genes required for autophagosome formation in mammalian cells. mTOR interacts with this complex in a nutrient dependent manner and phosphorylates Atg13 and ULK1.

基因ID

9776

NCBI登录号

NP_001136145

UniProt

O75143

途径

PI3K-Akt Signaling, Autophagy