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alpha Tubulin 抗体 (C-Term)

Name: alpha Tubulin 抗体 (C-Term)
Brand: antibodies-online
SKU: ABIN964563
Availability: OutOfStock

TUBA1 适用: 人, 小鼠, 大鼠, 小鸡, Cow WB, ELISA, IHC, FM 宿主: 小鼠 Monoclonal 17H11-F10 unconjugated

Scx is required for the expression of Fmod and Adamtsl2. (alpha Tubulin 抗体 (C-Term))

Immunofluorescence (IF) image for anti-alpha Tubulin (TUBA1) (C-Term) antibody (ABIN964563) (alpha Tubulin 抗体 (C-Term))

Immunofluorescence of Mouse monoclonal anti-Alpha Tubulin antibody. (alpha Tubulin 抗体 (C-Term))

Immunofluorescences of Mouse monoclonal anti-Alpha Tubulin antibody. (alpha Tubulin 抗体 (C-Term))

HeLa whole cell lysate (left lane) and HEK293 whole cell lysate (right lane) were loaded with 10 µg of lysate each. (alpha Tubulin 抗体 (C-Term))

Smad3 is required for TGF-β to regulate expression of Fmod and Adamtsl2. (alpha Tubulin 抗体 (C-Term))

TGF-β signaling regulates noncanonical pathways in the sclerotome. (alpha Tubulin 抗体 (C-Term))

AKT and p38 are not required for TGF-β-mediated regulation of fibrous tissue markers. (alpha Tubulin 抗体 (C-Term))

ERK is required for fibrous tissue marker regulation but not required to inhibit chondrogenesis. (alpha Tubulin 抗体 (C-Term))

13 images

(6 references)

产品编号 ABIN964563

发货至: 中国

Datasheet as PDF

抗原 See all alpha Tubulin (TUBA1) 抗体

alpha Tubulin (TUBA1)
抗原表位
16

10

6

5

4

4

4

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
C-Term
适用
102

76

67

22

18

15

14

12

8

5

5

4

4

4

4

4

3

3

3

3

3

3

3

3

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1
人, 小鼠, 大鼠, 小鸡, Cow
宿主
56

52

8

2

2

1

1

1
小鼠
Compatible Secondaries
克隆类型
68

52

2
单克隆
标记
85

11

6

3

2

2

2

2

2

2

2

1

1

1

1
This alpha Tubulin antibody is un-conjugated
应用范围
97

44

37

35

34

34

26

15

7

7

4

2

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Fluorescence Microscopy (FM)

特异性

Anti-Tubulin Loading Control Antibody was purified Protein A chromatography. This Loading Control antibody is directed against alpha tubulin. A BLAST analysis was used to suggest antibody reactivity with alpha tubulin from a wide range of organisms, including avian, mammalian aquatic, parasitic and alga sources based on 100% homology for the immunogen sequence. Cross reactivity will occur with all isoforms of alpha tubulin. Such broad reactivity makes this antibody useful as an excellent loading control.

交叉反应

小鼠, 大鼠, 小鸡, 绵羊

产品特性

Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein consisting of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter. Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle. This antibody makes an excellent loading control.

过滤

Sterile filtered

免疫原

Anti-Tubulin Loading Control Antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues near the C terminal end of of human alpha tubulin protein.

克隆位点

17H11-F10

亚型

IgG1 kappa

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anti-alpha Tubulin (TUBA1) antibody (FITC)
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anti-alpha Tubulin (TUBA1) antibody (Biotin)
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anti-alpha Tubulin (TUBA1) antibody
TUBA1 适用: 人, 小鼠, 大鼠, Pig, Nicotiana tabacum, 犬 WB, ELISA, IHC (p), IP, ICC 宿主: 小鼠 Monoclonal TU-16 unconjugated

anti-alpha Tubulin (TUBA1) (Internal Region) antibody
TUBA1 适用: 人, 小鼠, 大鼠, 小鸡, Cow WB, ELISA 宿主: 兔 Polyclonal unconjugated

anti-alpha Tubulin (TUBA1) (AA 380-460) antibody
TUBA1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF 宿主: 兔 Polyclonal unconjugated

anti-alpha Tubulin (TUBA1) (acLys40) antibody
TUBA1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-alpha Tubulin (TUBA1) antibody
TUBA1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-alpha Tubulin (TUBA1) antibody
TUBA1 适用: 人, 小鼠, 大鼠, Pig, Cow, Saccharomyces cerevisiae, 猴, Hamster, 犬, 豚鼠, 山羊, Sea Urchin, Amphibian, Kangaroo WB, IHC, IHC (p), IP 宿主: 小鼠 Monoclonal DM1A unconjugated

anti-alpha Tubulin (TUBA1) (Subunit alpha) antibody
TUBA1 适用: 人, Saccharomyces cerevisiae, 哺乳动物, Avian, Drosophila melanogaster, Fungus WB, ELISA, IHC, IHC (p), IHC (fro), RIA 宿主: 大鼠 Monoclonal YOL1-34 unconjugated

应用备注

Anti-Tubulin Antibody has been tested for use in ELISA, immunohistochemistry, immunofluorescence microscopy and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~50 kDa in size corresponding to alpha tubulin by western blotting in most cell lysates or extracts.

说明

Gene Name: TUBA1B

限制

仅限研究用
状态

Liquid

浓度

1.0 mg/mL

缓冲液

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

储存液

Sodium azide

注意事项

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C/-20 °C

储存方法

Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

有效期

12 months
Clayton, Ban, Liu, Serra: "Canonical and noncanonical TGF-β signaling regulate fibrous tissue differentiation in the axial skeleton." in: Scientific reports, Vol. 10, Issue 1, pp. 21364, (2021) (PubMed).

Chavez, Coricor, Perez, Seo, Serra: "SOX9 protein is stabilized by TGF-β and regulates PAPSS2 mRNA expression in chondrocytes." in: Osteoarthritis and cartilage, Vol. 25, Issue 2, pp. 332-340, (2018) (PubMed).

Patel, Blaauboer, Tucker, Mansouri, Ruiz-Moreno, Hamann, Schumann, Opitz, Jin: "The Common R71H-G230A-R293Q Human TMEM173 Is a Null Allele." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 198, Issue 2, pp. 776-787, (2017) (PubMed).

Roy, Jerman, Jozsef, McNamara, Onyekaba, Sun, Marin: "Palmitoylation of the ciliary GTPase ARL13b is necessary for its stability and its role in cilia formation." in: The Journal of biological chemistry, Vol. 292, Issue 43, pp. 17703-17717, (2017) (PubMed).

Laitem, Zaborowska, Isa, Kufs, Dienstbier, Murphy: "CDK9 inhibitors define elongation checkpoints at both ends of RNA polymerase II-transcribed genes." in: Nature structural & molecular biology, Vol. 22, Issue 5, pp. 396-403, (2015) (PubMed).

Blaauboer, Gabrielle, Jin: "MPYS/STING-mediated TNF-α, not type I IFN, is essential for the mucosal adjuvant activity of (3'-5')-cyclic-di-guanosine-monophosphate in vivo." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 192, Issue 1, pp. 492-502, (2014) (PubMed).
抗原

alpha Tubulin (TUBA1)

别名

Alpha-Tubulin (TUBA1 产品)

别名

Tuba1 antibody, K-ALPHA-1 antibody, Tuba-1 antibody, cb944 antibody, fb22g06 antibody, tuba1 antibody, wu:fb22g06 antibody, Calpha1 antibody, tubulin, alpha 1A antibody, tubulin alpha 1b antibody, tubulin, alpha 1b antibody, tubulin, alpha 1B antibody, Tuba1a antibody, TUBA1B antibody, tuba1b antibody, Tuba1b antibody

背景

Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein consisting of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter. Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle. This antibody makes an excellent loading control.
Synonyms: Tubulin alpha-1B chain, Tubulin alpha-ubiquitous chain, Alpha-tubulin ubiquitous Tubulin K-alpha-1, TUBA1B, tubulin loading control

基因ID

17986283

UniProt

P68363

途径

Microtubule Dynamics