Protein C Tag 抗体
WB, ELISA, FACS, IF, IP, ICC
宿主: 小鼠
Monoclonal
unconjugated
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- 抗原 See all Protein C Tag products
- Protein C Tag
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Protein C Tag antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC)
- 品牌
- THE™
- 特异性
- THETM Protein C Tag Antibody, mAb, Mouse recognizes N-terminal and C-terminal Protein C tagged fusion proteins.
- 产品特性
- The THETM Protein C Tag Antibody, mAb, Mouse is produced from the hybridoma resulting from fusion of SP2/0-Ag14 myeloma and B-lymphocytes obtained from mouse immunized with Protein C tag peptide conjugated to KLH.
- 纯化方法
- Protein A affinity column
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anti-Protein C Tag antibody
WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Protein C Tag antibodyWB, EIA 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 0.1-0.5 µg/mL.
Immunoprecipitation: 1-5 µg/mL.
Flow cytometry: 1-3 µg for 1 x 10^6 cells.
ICC/IF: 1-3 µg/mL.
ELISA: 0.001-0.002 µg/mL. - 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Reconstitute the lyophilized product with deionized water (or equivalent) to make antibody concentration of 0.5 mg/mL.
- 缓冲液
- PBS, pH 7.4, containing 0.02 % Sodium azide.
- 储存液
- Sodium azide
- 注意事项
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- 注意事项
- Avoid repeated freezing and thawing cycles.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- THETM Protein C Tag Antibody, mAb, Mouse should be stored lyophilized until use. It remains stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C or for up to 12 months at -20°C or below.
- 有效期
- 12 months
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- 抗原
- Protein C Tag
- Abstract
- Protein C Tag 产品
- 物质类
- Tag
- 背景
- Protein C is a Vitamin K-dependent plasma zymogen that is activated by proteolytic cleavage of the thrombin-thrombomodulin complex to form an anticoagulant enzyme. Protein C Tag is a 12 amino acid sequence EDQVDPRLIDGK, which encodes residues 6 through 17 of the heavy chain of the protein. Protein C Tag Antibody is a useful tool in analysis of Protein C fusion proteins.
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