Strep Tag II 抗体 (FITC)
IF, ICC, FACS
宿主: 小鼠
Monoclonal
5A9F9
FITC
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- 抗原
- Strep Tag II
- 适用
- 请咨询
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- FITC
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应用范围
- Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)
- 品牌
- THE™
- 序列
- NWSHPQFEK
- 特异性
- THETM NWSHPQFEK Tag Antibody [FITC], mAb, Mouse recognizes N-terminal and C-terminal Strep II tagged fusion proteins.
- 产品特性
- The THETM NWSHPQFEK Tag Antibody [FITC], mAb, Mouse is THETM NWSHPQFEK Tag Antibody conjugated with FITC. The conjugated antibody is suitable for detecting the expression level of NWSHPQFEK (Strep II tag) tagged fusion proteins.
- 纯化方法
- Protein A affinity column
- 免疫原
- NWSHPQFEK peptide conjugated - KLH
- 克隆位点
- 5A9F9
- 亚型
- IgG1
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anti-Strep Tag II antibody
WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Strep Tag II antibody (Biotin)WB, ELISA 宿主: 小鼠 Monoclonal 5A9F9 Biotin
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- 应用备注
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Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
FACS: 1-3 µg for 1 x 10^6 cells
ICC/IF: 1-3 µg/mL - 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Reconstitute the lyophilized product with deionized water (or equivalent) to make antibody concentration of 0.5 mg/mL.
- 缓冲液
- PBS, pH 7.4, containing 10 mg/mL BSA and 0.02 % Sodium azide.
- 储存液
- Sodium azide
- 注意事项
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- 注意事项
- Avoid repeated freezing and thawing cycles.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below.
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- 抗原
- Strep Tag II
- 物质类
- Tag
- 背景
- Streptavidin is a tetrameric protein purified from Streptomyces avidinii. It has wide use in numerous molecular biological protocols dues to its strong affinity for biotin. Strep II tag (NWSHPQFEK) is a nine amino acid peptide with high specificity and affinity towards streptavidin.
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