GST 抗体
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- 抗原 See all GST 抗体
- GST (Glutathione S Transferase (GST))
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适用
- Schistosoma japonicum
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This GST antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunoprecipitation (IP)
- 品牌
- THE™
- 特异性
- THETM Anti-GST Monoclonal Antibody (Mouse) recognizes C-terminal, N-terminal, and internal tagged GST fusion proteins.
- 纯化方法
- Protein A affinity column
- 免疫原
- Recombinant GST protein (26 kD)
- 克隆位点
- 2F10B9
- 亚型
- IgG1
- Top Product
- Discover our top product GST Primary Antibody
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anti-Glutathione S Transferase (GST) antibody
GST 适用: Schistosoma japonicum WB, IP, IF 宿主: 兔 Polyclonal unconjugated
anti-Glutathione S Transferase (GST) antibodyGST 适用: Schistosoma japonicum WB, ELISA, IP, DB 宿主: 山羊 Polyclonal unconjugated
anti-Glutathione S Transferase (GST) antibodyGST 适用: 标记 WB 宿主: 山羊 Polyclonal unconjugated
anti-Glutathione S Transferase (GST) antibodyGST 适用: Schistosoma japonicum WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Glutathione S Transferase (GST) antibodyGST 适用: Schistosoma japonicum IA 宿主: 小鼠 Monoclonal 3D4 unconjugated
anti-Glutathione S Transferase (GST) antibody (FITC)GST 适用: Schistosoma japonicum FACS 宿主: 兔 Polyclonal FITC
anti-Glutathione S Transferase (GST) antibody (Biotin)GST 适用: Schistosoma japonicum WB, ELISA, IHC, IM 宿主: 山羊 Polyclonal Biotin
anti-Glutathione S Transferase (GST) (AA 1-218) antibodyGST 适用: Various Species WB, IP, DB 宿主: 兔 Polyclonal unconjugated
anti-Glutathione S Transferase (GST) (AA 1-218) antibodyGST 适用: Various Species WB, IP, DB 宿主: 小鼠 Monoclonal unconjugated
anti-Glutathione S Transferase (GST) antibodyGST 适用: Schistosoma japonicum WB, ELISA 宿主: 小鼠 Monoclonal 3E2 unconjugated
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- 应用备注
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Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/mL
Western blot: 0.1-1 µg/mL
Western blot using ONE-HOUR Western Kit: For quick results, ONE-HOUR WesternTM Kit is recommended. 1.2 µg of this antibody is mixed with diluted WB solution (dilute 2 mL WB with 2 mL of PBST).
Immunoprecipitation (IP): 1 µg/mL
Other applications: user-optimized - 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/mL.
- 缓冲液
- PBS, pH 7.4, containing 0.02 % Sodium azide
- 储存液
- Sodium azide
- 注意事项
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
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- 抗原
- GST (Glutathione S Transferase (GST))
- 别名
- Glutathione-S-Transferase (GST) (GST 产品)
- 别名
- GST antibody, GST 13-13 antibody, GST13 antibody, GST13-13 antibody, GSTK1-1 antibody, hGSTK1 antibody, 1500002K10Rik antibody, Gst antibody, Ptgds2 antibody, glutathione S-transferase kappa 1 antibody, microsomal glutathione S-transferase 1 antibody, hematopoietic prostaglandin D synthase antibody, GSTK1 antibody, Mgst1 antibody, Hpgds antibody
- 背景
- Schistosoma japonicum glutathione S-transferase (GST) is a 26,000 Da enzyme that conjugates reduced glutathione to hydrophobic electrophiles. GST is an ideal fusion partner for production of foreign proteins in commercially available expression systems because it can be expressed in high levels in E. coli. The fusion proteins can be purified to homogenity in a single step as the GST portion of the protein binds tightly to immobilized glutathione. Elution can be accomplished using free glutathione. The GST portion of the fusion protein can be separated from the protein of interest by using site-specific proteases. Antibodies directed against GST can be helpful in detecting the fusion protein during purification and to detect cleavage of GST from the protein of interest.
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