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beta Actin 抗体

Name: beta Actin 抗体
SKU: ABIN962807
Availability: OutOfStock
Rating: 5 (1 reviews)

ACTB 适用: 人, 小鼠, 小鸡, 兔, Pig, Cow, Hamster, 山羊, 鱼 WB, ELISA 宿主: 小鼠 Monoclonal 2D1D10 unconjugated

Western Blotting validation image for anti-Actin, beta (ACTB) antibody (ABIN962807) (beta Actin 抗体)

Lane 1: 20 µg Hela cell lysate Lane 2: 20 µg sp2/0 cell lysate Lane 3: 20 µg goat muscle lysate Lane 4: 20 µg rabbit muscle lysate Lane 5: 20 µg chicken muscle lysate Lane 6: 20 µg CHO cell lysate Lane 7: 20 µg fish muscle lysate Primary antibody: 1 µg/mL Anti-beta-actin Monoclonal Antibody (Mouse) (ABIN396859) Secondary antibody: Goat Anti-Mouse IgG (H&L) [HRP] Polyclonal Antibody (ABIN398387, 1: 20,000) (beta Actin 抗体)

4 images

(2 references)

(1 validation)

产品编号 ABIN962807

发货至: 中国

Datasheet as PDF

抗原 See all beta Actin (ACTB) 抗体

beta Actin (ACTB) (Actin, beta (ACTB))
适用
192

137

120

64

51

43

37

36

32

25

20

20

12

10

9

7

7

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6

3

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3

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2

1

1

1

1

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1

1

1
人, 小鼠, 小鸡, 兔, Pig, Cow, Hamster, 山羊, 鱼
宿主
115

93

5

1

1
小鼠
Compatible Secondaries
克隆类型
114

99
单克隆
标记
129

14

13

11

4

4

4

4

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2

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1

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This beta Actin antibody is un-conjugated
应用范围
175

77

60

51

35

30

27

22

13

13

6

6

6

5

5

2

1

1
Western Blotting (WB), ELISA

特异性

THE^TM beta Actin Antibody, mAb, Mouse reacts with mouse, rabbit, chicken, human, hamster, cow, goat, fish, and pig. It has not yet been tested in other species.

交叉反应（详细）

Cross reactivity in other species has not yet been tested.

纯化方法

Protein G affinity column

免疫原

A synthetic peptide DDDIAALVVDNGSG coupled - KLH

克隆位点

2D1D10

亚型

IgG

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anti-Actin, beta (ACTB) (AA 1-25) antibody
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anti-Actin, beta (ACTB) (AA 1-375) antibody
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anti-Actin, beta (ACTB) antibody
ACTB 适用: 人, 小鼠, 大鼠 WB, IF, IHC (p), FACS, DNA Mic 宿主: 小鼠 Monoclonal 137CT26-1-1 unconjugated

anti-Actin, beta (ACTB) (AA 1-50) antibody
ACTB 适用: 人 WB, ELISA, IHC, IF, IP, FACS 宿主: 小鼠 Monoclonal 1D12E12B5 unconjugated

应用备注

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.1-1.0 µg/mL
Western blot: 1.0 µg/mL
Other applications: user-optimized

限制

仅限研究用
生效 #102832 (Western Blotting)

by

Dr. Randy Brutkiewicz Laboratory, Department of Microbiology and Immunology, Indiana University School of Medicine

No.

#102832

日期

2018.02.20

抗原

ACTB

Lot Number

Method validated

Western Blotting

Positive Control

lysates from HEK293 cells untransfected or transfected with human MR1 cDNA

Negative Control

Notes

Passed. ABIN962807 recognizes human beta actin in HEK293 cell lysates.

Validation Images

Lysates from human MR1-expressing HEK293 cells (MR1) and vector control cells (Vc) were resolved on a 10% SDS-PAGE gel for Western blotting analysis using antibodies specific for MR1 (ABIN1537116) and beta-actin (ABIN962807).
Full Methods
Primary Antibody

ABIN962807

Secondary Antibody

goat anti-rabbit Dye-IR800 conjugated antibody (Advansta, R-05060-250, lot 17083179)

Full Protocol

Grow HEK293 cells in DMEM medium (Lonza, 12-614F, lot 0000618582) supplemented with serum (Hyclone, SH30071.03, lot AAG205460) and antibiotics (Hyclone, SV30010, lot J150013), at 37°C and 5% CO₂ dish to 70-90% confluency.
Transfect cells with pCDNA 3.1 neo (-) (Invitrogen) containing human MR1 cDNA (Genecopoeia) using Polyethylenimine (Polysciences, 23966) following the manufacturer´s instructions.
Lyse cells in cold lysis buffer (10mM Tris pH7.4, 150mM NaCl, 0.5mM EDTA, 2% CHAPS).
Determine total protein content of the lysates using Commassie Protein Assay Reagent (Thermo Scientific, 1856209, lot NL179252).
Denature 200µg total protein for 5min at 95°C in 20µl Laemmli SDS sample buffer and subsequently separate them on a SDS-PAGE gel using Acrylamide/Bis Premixed (Bio-Rad, 61-0125, lot 260000477) for 2-3h at 100V.
Transfer proteins onto PVDF membrane (Millipore, IPVH00010, lot K5AA6843U) with a Western blotting system for ON at 4°C at 150mA.
Block the membrane with blocking buffer (2% BSA/PBS/0.05%Tween-20) for 1h at RT.
Incubate membrane with:
loading control rabbit anti beta-actin (antibodies-online, ABIN962807) diluted 1:500 in blocking buffer ON at 4°C.
primary rabbit anti-MR1 antibody (antibodies-online, ABIN1537116, lot SA111213CH) diluted 1:1000 in blocking buffer ON at 4°C.
Wash membrane 3x for 10min with PBS/0.05%Tween-20.
Incubate membrane with secondary goat anti-rabbit Dye-IR800 conjugated antibody (Advansta, R-05060-250, lot 17083179) diluted 1:10000 in PBS/0.05% Tween-20 for 1h at RT.
Wash membrane 3x for 10min with PBS/0.05% Tween-20.
Reveal protein bands using an Odyssey imaging system (LI-COR Biosciences).

Experimental Notes

The beta Actin antibody ABIN962807 reveals a protein of the expected molecular weight of beta actin in lysates of human HEK293 cells.
状态

Lyophilized

溶解方式

Reconstitute the lyophilized antibody with deionized water to the final concentration of 0.5 mg/mL.

缓冲液

PBS, pH 7.4, containing 0.02 % Sodium azide, without BSA

储存液

Sodium azide

注意事项

WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

储存条件

4 °C/-20 °C

储存方法

The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Zhao, Mo, Li, Zou, Cheng, Sun, Xiong, Guan, Lei: "NOTCH-induced aldehyde dehydrogenase 1A1 deacetylation promotes breast cancer stem cells." in: The Journal of clinical investigation, (2014) (PubMed).

Srivastava, Reinoso, Singh, Fernandez-Bueno, Martino, Garcia-Gutierrez, Pastor, Corell: "Flow cytometry assessment of the purity of human retinal pigment epithelial primary cell cultures." in: Journal of immunological methods, Vol. 389, Issue 1-2, pp. 61-8, (2013) (PubMed).
抗原

beta Actin (ACTB) (Actin, beta (ACTB))

别名

beta Actin (ACTB 产品)

别名

BRWS1 antibody, PS1TP5BP1 antibody, ACTA1 antibody, Actx antibody, E430023M04Rik antibody, beta-actin antibody, ps1tp5bp1 antibody, act-2 antibody, actb antibody, B-actin antibody, LOC100174883 antibody, LOC100304816 antibody, A antibody, ACT5C antibody, Ac5C antibody, Act antibody, Act-5C antibody, Act5 antibody, Act5c antibody, Actin antibody, Actin/BAP47 antibody, Actin5C antibody, BAP47 antibody, Bap47 antibody, CG4027 antibody, Dmel\\CG4027 antibody, M32055 antibody, T11 antibody, act antibody, act 5C antibody, act5C antibody, actin antibody, actin5C antibody, anon-EST:fe2D2 antibody, beta-actin/Bap47 antibody, cyt5C antibody, l(1)G0009 antibody, l(1)G0010 antibody, l(1)G0025 antibody, l(1)G0079 antibody, l(1)G0117 antibody, l(1)G0177 antibody, l(1)G0245 antibody, l(1)G0330 antibody, l(1)G0420 antibody, l(1)G0486 antibody, Bact antibody, ACT-5 antibody, ACTB antibody, B-ACTZF antibody, actba antibody, bact antibody, bactin1 antibody, bactzf antibody, wu:fd18f05 antibody, A26C1B antibody, POTE2alpha antibody, POTEACTIN antibody, Beta-actin antibody, actin beta antibody, actin, beta antibody, cytoplasmic actin OlCA1 antibody, beta-actin antibody, Actin 5C antibody, actin gamma 1 antibody, actin, beta 1 antibody, actin, beta L homeolog antibody, beta actin antibody, POTE ankyrin domain family member F antibody, actin, beta like 2 antibody, actin, cytoplasmic 1 antibody, ACTB antibody, Actb antibody, actb antibody, LOC100174883 antibody, LOC100304816 antibody, Act5C antibody, ACTG1 antibody, actb1 antibody, actb.L antibody, LOC100136352 antibody, POTEF antibody, ACTBL2 antibody, LOC109073280 antibody

背景

Beta-actin is one of the six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. As a consequence, it has been difficult to produce potent antisera against this protein. Therefore, the availability of monoclonal antibodies to beta-actin provides a useful and specific tool in the study of the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton.

途径

Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction