Collagen IV 抗体
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- 抗原 See all Collagen IV (COL4) 抗体
- Collagen IV (COL4) (Collagen, Type IV (COL4))
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Collagen IV antibody is un-conjugated
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应用范围
- ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)
- 品牌
- IHC-plus™
- 特异性
- Human Type IV collagen. Cross-reactivity: Human collagen type I: 1 % Human collagen type II: <0.5 %, Human collagen type III: <0.5 %, Human collagen type IV: 100 %, Human collagen type V: 7 %, Human fibronectin: <0.5 %, Human albumin: <0.5 %, Human immunoglobulin <0.5 %.
- 纯化方法
- Affinity purified
- 免疫原
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Human placental collagen type IV.
Type of Immunogen: Purified protein - 亚型
- IgG
- Top Product
- Discover our top product COL4 Primary Antibody
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- 应用备注
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Approved: ELISA (1:3000), IF, IHC, IHC-Fr, IHC-P (1:100)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working dilution for LS-B3654 was determined to be 1:100. Suitable for use in ELISA, Indirect immunofluorescence, indirect immunohistochemistry and Dot Blotting (not suitable for Western Blot). ELISA: 1:3000. Indirect immunofluorescent visualization of collagen type IV on cryostat sections of human tissue: 1:20-1:40. Indirect Immunohistochemistry: 1:20-1:40. fresh frozen or acetone fixed specimens. Dot Blotting: 1:300. not recommended for Western Blots.
Not recommended for: WB - 说明
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Target Species of Antibody: Human
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, mannitol, dextran, no preservatives.
- 储存液
- Without preservative
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- 4°C or -20°C, Avoid freeze-thaw cycles.
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- 抗原
- Collagen IV (COL4) (Collagen, Type IV (COL4))
- 别名
- Collagen IV (COL4 产品)
- 别名
- collagen, type IV antibody, Let-2 antibody
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