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CPY 抗体

Rockland 100-401-135 CPY 适用: Saccharomyces cerevisiae WB, ELISA 宿主: 兔 Polyclonal unconjugated
Rockland
产品编号 ABIN96043
Supplier Product No.: 100-401-135
发货至: 中国
  • 抗原 See all CPY 抗体
    CPY (Carboxypeptidase Y (CPY))
    适用
    Saccharomyces cerevisiae
    宿主
    • 11
    克隆类型
    • 11
    多克隆
    标记
    • 6
    • 4
    • 1
    This CPY antibody is un-conjugated
    应用范围
    • 11
    • 8
    • 7
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 1
    Western Blotting (WB), ELISA
    Supplier Product No.
    100-401-135
    Supplier
    Rockland
    原理
    Carboxypeptidase Y Antibody
    交叉反应 (详细)
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified Carboxypeptidase Y [Baker's Yeast].
    产品特性
    Synonyms: rabbit anti-Carboxypeptidase Y antibody, Carboxypeptidase YSCY antibody, CPY1 antibody, LBC1 antibody, PRC1 antibody, Vacuolar carboxypeptidase Y antibody, YMR297W antibody
    纯化方法
    This product was prepared from monospecific antiserum by a delipidation and defibrination.
    免疫原

    Immunogen: Carboxypeptidase Y [Baker's Yeast]

    Immunogen Type: Native Protein

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  • 应用备注

    Application Note: Anti-Carboxypeptidase Y has been tested by western blot and is suitable to be assayed against 1.0 μg of Carboxypeptidase Y [Baker's Yeast] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:1,000 to 1:3,000 of the reconstitution concentration is suggested for this product.

    Western Blot Dilution: 1:500 - 1:3,000

    ELISA Dilution: 1:5,000 - 1:25,000

    Other: User Optimized

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式

    Reconstitution Volume: 2.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    浓度
    90 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    有效期
    12 months
  • Martinez Benitez, Stolz, Becher, Wolf: "Mnl2, a novel component of the ER associated protein degradation pathway." in: Biochemical and biophysical research communications, (2011) (PubMed).

    Ohashi, Munro: "Membrane delivery to the yeast autophagosome from the Golgi-endosomal system." in: Molecular biology of the cell, Vol. 21, Issue 22, pp. 3998-4008, (2011) (PubMed).

    von Numers, Survila, Aalto, Heino, Palva, Li: "Requirement of a Homolog of Glucosidase II {beta}-Subunit for EFR-Mediated Defense Signaling in Arabidopsis thaliana." in: Molecular plant, (2010) (PubMed).

  • 抗原
    CPY (Carboxypeptidase Y (CPY))
    别名
    Carboxypeptidase Y (CPY 产品)
    背景
    Background: Carboxypeptidase Y is involved in degradation of small peptides. It digests preferentially peptides containing an aliphatic or hydrophobic residue in P1' position, as well as methionine, leucine or phenylalanine in P1 position of ester substrate. Carboxypeptidase that catalyzes the release of a C-terminal amino acid with broad specificity. It is inhibited by ZPCK.
    基因ID
    855343
    NCBI登录号
    NP_014026
    UniProt
    P00729
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