Cross reactivity against Carbonic Anhydrase II from other tissues and species may occur but have not been specifically determined.
纯度
Anti-CARBONIC ANHYDRASE II Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Carbonic Anhydrase II [Bovine Erythrocytes].
内毒素水平
Low Endotoxin : No
免疫原
Carbonic Anhydrase II [Bovine Erythrocytes] Immunogen Type: Native Protein
Anti CARBONIC ANHYDRASE II Antibody has been assayed against 1.0 µg of Carbonic Anhydrase II [Bovine Erythrocytes] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) [code #611-1302] and TMBE [code # TMBE-1000] as a substrate for 30 minutes at room temperature. ELISA Dilution: 1:20.000 - 1:100.000 Western Blot Dilution: 1:2.000 - 1:10.000
限制
仅限研究用
状态
Liquid
浓度
1 mg/mL
缓冲液
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
注意事项
Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
储存条件
-20 °C
储存方法
Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
有效期
Expiration date is one (1) year from date of opening.
CARBONIC ANHYDRASE II Antibody is specific for carbonic anhydrase II. Carbonic anhydrases (or carbonate dehydratases) are a family of metalloenzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and Hydrogen (or vice versa). This reversible reaction occurs slowly in the absence of the carbonic anhydrase catalyst. The active site of most carbonic anhydrases contains a zinc ion cofactor. One of the functions of the enzyme in animals is to interconvert carbon dioxide and bicarbonate to maintain acid-base balance in blood and other tissues, and to help transport carbon dioxide out of tissues. Synonyms: Carbonate dehydratase II antibody, Carbonic anhydrase 2 antibody, Carbonic anhydrase B antibody, Carbonic anhydrase C antibody, Carbonic anhydrase II antibody, Carbonic dehydratase antibody