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FITC 抗体

FITC ELISA, WB 宿主: 小鼠 Monoclonal NI 239 unconjugated
产品编号 ABIN958555
发货至: 中国
  • 抗原 See all FITC products
    FITC (Fluorescein Isothiocyanate (FITC))
    适用
    请咨询
    宿主
    • 40
    • 17
    • 16
    • 10
    • 8
    小鼠
    克隆类型
    • 49
    • 41
    单克隆
    标记
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    • 7
    • 5
    • 2
    • 1
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    This FITC antibody is un-conjugated
    应用范围
    • 55
    • 38
    • 31
    • 17
    • 15
    • 14
    • 14
    • 5
    • 5
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    • 3
    • 2
    • 2
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    • 1
    • 1
    • 1
    ELISA, Western Blotting (WB)
    特异性
    FITC. The reactivity of the antiserum is directed to the FITC molecule as tested in direct binding enzyme immunoassay, ELISA and immunoblotting. Cross-reactivity This antiserum has not been tested for cross-reactivity with other fluorescent compounds.
    纯化方法
    Purified IgG
    免疫原
    FITC

    Type of Immunogen: Purified protein
    克隆位点
    NI 239
    亚型
    IgG1
  • 应用备注
    Approved: ELISA, WB

    Usage: This product is intended for use to enhance the specific signal obtained with a monoclonal antibody or a polyclonal second antibody conjugated to FITC. The phenomenon of a weak reaction of a monoclonal antibody is e. g. well known in different analysis of vital peripheral blood mononuclear cells in suspensions by the expression of surface markers. A similar situation exists in solid phase assay systems (ELISA, blotting, DIBA) when used for the identification and /or quantitative determination of minute amounts of soluble specific antigens or antibodies. The sensitivity of the FITC hapten-anti-hapten system makes it a valuable alternative to the biotinavidin system. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200, in ELISA from 1:500 upwards, in Western blotting from 1:1000 upwards. These data should be interpreted as general recommendations only.
    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Sterile distilled water 0.5 ml
    浓度
    Lot specific
    缓冲液
    Lyophilized from PBS, pH 7.2
    注意事项
    Avoid repeat freeze-thaw cycles.
    储存条件
    4 °C,-20 °C
    储存方法
    Long term: -20°C
    Short term: +4°C. Avoid repeat freeze-thaw cycles.
  • 抗原
    FITC (Fluorescein Isothiocyanate (FITC))
    别名
    FITC (FITC 产品)
    物质类
    Chemical
    背景
    Name/Gene ID: FITC

    Synonyms: FITC
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