FITC. The reactivity of the antiserum is directed to the FITC molecule as tested in direct binding enzyme immunoassay, ELISA and immunoblotting. Cross-reactivity This antiserum has not been tested for cross-reactivity with other fluorescent compounds.
Usage: This product is intended for use to enhance the specific signal obtained with a monoclonal antibody or a polyclonal second antibody conjugated to FITC. The phenomenon of a weak reaction of a monoclonal antibody is e. g. well known in different analysis of vital peripheral blood mononuclear cells in suspensions by the expression of surface markers. A similar situation exists in solid phase assay systems (ELISA, blotting, DIBA) when used for the identification and /or quantitative determination of minute amounts of soluble specific antigens or antibodies. The sensitivity of the FITC hapten-anti-hapten system makes it a valuable alternative to the biotinavidin system. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200, in ELISA from 1:500 upwards, in Western blotting from 1:1000 upwards. These data should be interpreted as general recommendations only.
限制
仅限研究用
状态
Lyophilized
溶解方式
Sterile distilled water 0.5 ml
浓度
Lot specific
缓冲液
Lyophilized from PBS, pH 7.2
注意事项
Avoid repeat freeze-thaw cycles.
储存条件
4 °C,-20 °C
储存方法
Long term: -20°C Short term: +4°C. Avoid repeat freeze-thaw cycles.