Human PLG / Plasminogen. The defined antibody reactivity is restricted to plasminogen as tested at the level of sensitivity of immuno-precipitation techniques. A single precipitin line is obtained in bidimensional electrophoresis, immunoelectrophoresis and double radial immunodiffusion (Ouchterlony) which shows a reaction of full identity with the purified immunogen. No precipitation is obtained with any other plasma protein or with plasminogen-deficient plasma. The antiserum may also react with plasmin conversion intermediates and with plasmin. Cross-reactivity The antiserum does not cross-react with any other human plasma proteins as tested in gel-diffusion techniques. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins, since homologous proteins of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail, however in double radial immunodiffusion ea reaction has been observed with bovine, dog and rhesus monkey.
Usage: This product is intended for use in precipitating techniques as electroimmunodiffusion (Laurell), immunoelectrophoresis, single and double radial immunodiffusion (Mancini, Ouchterlony), immunonephelometry, solid phase assays, as catching or detection antibody in ELISA. EDTA should be added to the plasma samples and all assay components to stabilize the proteins, to prevent activation of plasminogen in plasma samples epsilonaminocaproic acid may be added.
限制
仅限研究用
状态
Lyophilized
溶解方式
Sterile distilled water 1 ml
浓度
Lot specific
缓冲液
Lyophilized antiserum
注意事项
Avoid repeat freeze-thaw cycles.
储存条件
4 °C,-20 °C
储存方法
Long term: -20°C Short term: +4°C. Avoid repeat freeze-thaw cycles.