Biotin 抗体
(8 references)
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- 抗原 See all Biotin products
- Biotin
- 适用
- 请咨询
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Biotin antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
- Supplier Product No.
- 100-4198
- Supplier
- Rockland
- 原理
- Biotin Antibody
- 交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, biotinylated IgG and Biotinylated BSA.
- 产品特性
- Synonyms: rabbit anti-biotin antibody, rabbit anti biotin
- 纯化方法
- This product was prepared from monospecific antiserum by a delipidation and defibrination.
- 免疫原
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Immunogen: Biotin conjugated to Keyhole Limpet Hemocyanin (KLH)
Immunogen Type: Native Protein
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anti-Biotin antibody
WB, ELISA, FACS, IHC, IF, FM 宿主: 小鼠 Monoclonal 4C7-D5 unconjugated
anti-Biotin antibodyWB, FACS, IHC, IF, ISt, CM 宿主: 小鼠 Monoclonal Hyb-8 unconjugated
anti-Biotin antibodyIHC, ISt 宿主: 兔 Monoclonal BTN-2032R unconjugated Recombinant Antibody
anti-Biotin antibody (DyLight 488)WB, FACS, IF, FM, FLISA, DB, MA 宿主: 山羊 Polyclonal DyLight 488
anti-Biotin antibodyELISA, FACS 宿主: 小鼠 Monoclonal SB58c unconjugated
anti-Biotin antibodyELISA, FACS, IP, CyTOF 宿主: 小鼠 Monoclonal 1D4-C5 unconjugated
anti-Biotin antibody适用: 人 WB 宿主: 兔 Monoclonal unconjugated
anti-Biotin antibodyWB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Immunohistochemistry Dilution: User Optimized
Application Note: Anti-Biotin has been tested in ELISA and is suitable for immunoblotting (western or dot blot), immunoprecipitation and most immunological methods requiring high titer and specificity.
Western Blot Dilution: 1:10,000 - 1:25,000
ELISA Dilution: 1:100,000
Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 2.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 78 mg/mL
- 缓冲液
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Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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: "SKIP-HOPS recruits TBC1D15 for a Rab7-to-Arl8b identity switch to control late endosome transport." in: The EMBO journal, Vol. 39, Issue 6, pp. e102301, (2020) (PubMed).
: "Pseudomonas aeruginosa lectin LecB impairs keratinocyte fitness by abrogating growth factor signalling." in: Life science alliance, Vol. 2, Issue 6, (2020) (PubMed).
: "The ubiquitin-conjugating enzyme UBE2QL1 coordinates lysophagy in response to endolysosomal damage." in: EMBO reports, Vol. 20, Issue 10, pp. e48014, (2019) (PubMed).
: "Zebrafish models for nemaline myopathy reveal a spectrum of nemaline bodies contributing to reduced muscle function." in: Acta neuropathologica, Vol. 130, Issue 3, pp. 389-406, (2016) (PubMed).
: "Antisense-targeted immuno-EM localization of the pre-mRNA path in the spliceosomal C complex." in: RNA (New York, N.Y.), Vol. 18, Issue 7, pp. 1347-57, (2012) (PubMed).
: "Pathogenic bacteria target NEDD8-conjugated cullins to hijack host-cell signaling pathways." in: PLoS pathogens, Vol. 6, Issue 9, pp. e1001128, (2011) (PubMed).
: "Structural insights into NEDD8 activation of cullin-RING ligases: conformational control of conjugation." in: Cell, Vol. 134, Issue 6, pp. 995-1006, (2008) (PubMed).
: "Localization of the AP-3 adaptor complex defines a novel endosomal exit site for lysosomal membrane proteins." in: The Journal of cell biology, Vol. 164, Issue 7, pp. 1065-76, (2004) (PubMed).
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: "SKIP-HOPS recruits TBC1D15 for a Rab7-to-Arl8b identity switch to control late endosome transport." in: The EMBO journal, Vol. 39, Issue 6, pp. e102301, (2020) (PubMed).
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- 抗原
- Biotin
- Abstract
- Biotin 产品
- 物质类
- Chemical
- 背景
- Background: Biotin Antibody detects Biotin. Biotin is a water-soluble B-complex vitamin (vitamin B7). It is composed of a ureido (tetrahydroimidizalone) ring fused with a tetrahydrothiophene ring. A valeric acid substituent is attached to one of the carbon atoms of the tetrahydrothiophene ring. Biotin is a coenzyme for carboxylase enzymes, involved in the synthesis of fatty acids, isoleucine, and valine, and in gluconeogenesis. Biotin is necessary for cell growth, the production of fatty acids, and the metabolism of fats and amino acids. Anti-Biotin Antibody is ideal for investigators involved in Cell Signaling and Cell Biology research.
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