电话:
+86 (0512) 65829739
传真:
+86 (010) 6788 5057
电子邮件:
orders@antibodies-online.cn

Bilirubin Oxidase 抗体 (Biotin)

Rockland 200-106-212-0100 适用: Myrothecium verrucaria WB, IP, ELISA 宿主: 山羊 Polyclonal Biotin
Rockland
产品编号 ABIN95598
Supplier Product No.: 200-106-212-0100
发货至: 中国
  • 抗原 See all Bilirubin Oxidase products
    Bilirubin Oxidase
    适用
    Myrothecium verrucaria
    宿主
    • 4
    山羊
    克隆类型
    • 4
    多克隆
    标记
    • 2
    • 1
    • 1
    This Bilirubin Oxidase antibody is conjugated to Biotin
    应用范围
    • 4
    • 4
    • 3
    • 2
    • 1
    Western Blotting (WB), Immunoprecipitation (IP), ELISA
    Supplier Product No.
    200-106-212-0100
    Supplier
    Rockland
    原理
    Bilirubin Oxidase Antibody Biotin Conjugated
    交叉反应 (详细)
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Bilirubin Oxidase [Myrothecium verrucaria].
    产品特性
    Synonyms: goat anti-Bilirubin Oxidase biotin conjugation, goat anti-Bilirubin Oxidase Antibody biotin Conjugated
    纯化方法
    Anti-Bilirubin Oxidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
    免疫原

    Immunogen: Bilirubin Oxidase [Myrothecium verrucaria]

    Immunogen Type: Native Protein

    亚型
    IgG
  • 应用备注

    Application Note: Anti-Bilirubin Oxidase has been tested by western blot and is suitable to be assayed against 1.0 μg of Bilirubin Oxidase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:1,000 to 1:3,000 of the reconstitution concentration is suggested for this product.

    Western Blot Dilution: 1:500 - 1:2,500

    Immunoprecipitation Dilution: 1:100

    ELISA Dilution: 1:1,000 - 1:5,000

    Other: User Optimized

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    浓度
    10.0 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Sodium Azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
    有效期
    12 months
  • 抗原
    Bilirubin Oxidase
    Abstract
    Bilirubin Oxidase 产品
    背景
    Background: Anti-Bilirubin Oxidase antibody is an enzyme that increases the oxidation rate of bilirubin to biliverdin and other substrates. It is ideal for investigators interested in Metabolism or Signal Transduction research.
    UniProt
    Q12737
You are here:
客服