Vimentin 抗体
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- 抗原 See all Vimentin (VIM) 抗体
- Vimentin (VIM)
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适用
- 人, 小鼠
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宿主
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小鸡
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克隆类型
- 多克隆
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标记
- This Vimentin antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 特异性
- Recognizes Vimentin.
- 纯化方法
- Affinity Chromatography using a peptide column.
- 免疫原
- Chickens were immunized with three synthetic peptide KLH conjugated corresponding to different regions of the Vimentin gene product, but are shared between the Human (NP_003371) and Mouse (NP_035831) sequences. Production: After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 0.3 mg/ml. Finally, equal volumes of each of the three affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.
- 亚型
- Ig Fraction
- Top Product
- Discover our top product VIM Primary Antibody
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 浓度
- 0.3 mg/mL
- 缓冲液
- 10 mM PBS, pH 7.2 containing 0.02 % Sodium Azide as preservative.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store the antibody undiluted in the dark at 2-8 °C.
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- 抗原
- Vimentin (VIM)
- 别名
- Vimentin (VIM 产品)
- 背景
- Human vimentin is a 53,521 dalton protein (466 amino acids) expressed in many cell types of mesodermal origin, and is one of the intermediate filament types that form the cytoskeleton. In the embryonic nervous system, however, vimentin is expressed by migrating neural crest cells forming the PNS, and by neural stem cells of the CNS. In some forms of glioblastoma, vimenin is reexpressed by the tumor cells and serves as a general indicator of their undifferentiated state.Synonyms: VIM
- 基因ID
- 7431
- NCBI登录号
- NP_003371
- 途径
- Caspase Cascade in Apoptosis
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